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X-Ray Microanalysis of Human Cementum

Published online by Cambridge University Press:  07 July 2005

Marco Antonio Alvarez-Pérez ,
Octavio Alvarez-Fregoso ,
Jaime Ortiz-López  and
Higinio Arzate
Marco Antonio Alvarez-Pérez
Affiliation:
Laboratorio de Biología Celular y Molecular, Facultad de Odontología, UNAM, Cd. Universitaria, Coyoacán, México D.F. 04510, México
Octavio Alvarez-Fregoso
Affiliation:
Instituto de Investigación en Materiales, Universidad Nacional Autónoma de México, Cd. Universitaria, Coyoacán, México D.F. 04510, México
Jaime Ortiz-López
Affiliation:
Escuela Superior de Física y Matemáticas, Instituto Politécnico Nacional, México, D.F. 07730, México
Higinio Arzate
Affiliation:
Laboratorio de Biología Celular y Molecular, Facultad de Odontología, UNAM, Cd. Universitaria, Coyoacán, México D.F. 04510, México
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Abstract

An energy dispersive x-ray microanalysis study was performed throughout the total length of cementum on five impacted human teeth. Mineral content of calcium, phosphorous, and magnesium were determined with an electron probe from the cemento-enamel junction to the root apex on the external surface of the cementum. The concentration profiles for calcium, phosphorous, and magnesium were compared by using Ca/P and Mg/Ca atomic percent ratio. Our findings demonstrated that the Ca/P ratio at the cemento-enamel junction showed the highest values (1.8–2.2). However, the area corresponding to the acellular extrinsic fiber cementum (AEFC) usually located on the coronal one-third of the root surface showed a Ca/P media value of 1.65. Nevertheless, on the area representing the fulcrum of the root there is an abrupt change in the Ca/P ratio, which decreases to 1.3. Our results revealed that Mg2+ distribution throughout the length of human cementum reached its maximum Mg/Ca ratio value of 1.3–1.4 at.% around the fulcrum of the root and an average value of 0.03%. A remarkable finding was that the Mg/Ca ratio pattern distribution showed that in the region where the Ca/P ratio showed a decreasing tendency, the Mg/Ca ratio reached its maximum value, showing a negative correlation. In conclusion, this study has established that clear compositional differences exist between AEFC and cellular mixed stratified cementum varieties and adds new knowledge about Mg2+ distribution and suggests its provocative role regulating human cementum metabolism.

Keywords

cementumhydroxyapatitemineralizationcalciumphosphorousmagnesiumEDS
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 11 , Issue 4 , August 2005 , pp. 313 - 318
Copyright
© 2005 Microscopy Society of America

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