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Visualizing Biological Tissues: A Multiscale Workflow from Live Imaging to 3D Cryo-CLEM

Published online by Cambridge University Press:  03 December 2021

Marit de Beer ,
Rona Roverts ,
Xavier Heiligenstein ,
Edwin Lamers ,
Nico Sommerdijk  and
Anat Akiva
Marit de Beer
Affiliation:
Department of Biochemistry, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. Electron Microscopy Center, Radboudumc Technology Center Microscopy, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
Rona Roverts
Affiliation:
Department of Biochemistry, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. Electron Microscopy Center, Radboudumc Technology Center Microscopy, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
Xavier Heiligenstein
Affiliation:
CryoCapCell, Kremlin-Bicêtre, France
Edwin Lamers
Affiliation:
Carl Zeiss BV, Breda, The Netherlands
Nico Sommerdijk
Affiliation:
Department of Biochemistry, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. Electron Microscopy Center, Radboudumc Technology Center Microscopy, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands
Anat Akiva
Affiliation:
Department of Biochemistry, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. Electron Microscopy Center, Radboudumc Technology Center Microscopy, Radboud Institute of Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands

Abstract

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Type
Soft Matter and Biological Specimens
Information
Microscopy and Microanalysis , Volume 27 , Supplement S2: Proceedings of CISCEM 2021 - the 5th Conference on In-Situ and Correlative Electron Microscopy , November 2021 , pp. 11 - 12
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Creative Commons
Creative Common License - CCCreative Common License - BYCreative Common License - NCCreative Common License - ND
This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

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Heiligenstein, X et al. , Methods in Cell Biology, ed. Müller-Reichert, T & Verkade, P, Academic Press 162 (2021), p. 115.Google Scholar
Vidavsky, N et al. , Journal of Structural Biology 196 (2016), p. 487.10.1016/j.jsb.2016.09.016CrossRefGoogle Scholar
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MdB, RR, NS and AA were supported by the European Research Council (ERC) Advanced Investigator grant (H2020-ERC-2017-ADV-788982-COLMIN), AA is also supported by a VENI grant of the Netherlands Scientific Organization NWO (VI.Veni.192.094).Google Scholar