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The Ultrastructure of Skeletal Muscle Capillaries of Streptozotocin Diabetic Rats and the Therapeutic Effect of Benfluorex

Part of: Micrographia Collection

Published online by Cambridge University Press:  07 October 2022

Nursel Gül Open the ORCID record for Nursel Gül [Opens in a new window] ,
Suna Cebesoy ,
Nesrin Özsoy ,
Hakan Eskizengin  and
Çiğdem Özer
Nursel Gül*
Affiliation:
Faculty of Sciences, Biology Department, Ankara University, Tandogan, Ankara 06100, Turkey
Suna Cebesoy
Affiliation:
Faculty of Sciences, Biology Department, Ankara University, Tandogan, Ankara 06100, Turkey
Nesrin Özsoy
Affiliation:
Faculty of Sciences, Biology Department, Ankara University, Tandogan, Ankara 06100, Turkey
Hakan Eskizengin
Affiliation:
Faculty of Sciences, Biology Department, Ankara University, Tandogan, Ankara 06100, Turkey
Çiğdem Özer
Affiliation:
School of Medicine, Physiology Department, Gazi University, Besevler, Ankara 06500, Turkey
*
*Corresponding author: Nursel Gül, E-mail: [email protected]
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Abstract

Diabetes mellitus is a serious disease worldwide and causes other associated diseases. In this study, we observed the effect of streptozotocin (STZ)-induced diabetes and benfluorex treatment on muscular capillary ultrastructure. Adult male rats were used as the test subjects and each individual was intraperitoneally injected with one dose of STZ (45 mg/kg) to induce diabetes. Doses (50 mg/kg) of benfluorex were given to the subjects with tap water by intragastric gavage application once daily for 21 days. At the end of day 21, muscle tissues were obtained from animals and examined under transmission electron microscopy. From the data obtained with the electron microscope, it was observed that the control group had typical continuous capillary vascular structures in their muscles, while STZ caused disruptive disorder of the muscle cells in the capillary wall of the STZ-diabetic group. Additionally, the thickening of the basement membrane around endothelial cells, loss of mitochondrial crista in the muscle cells, enlarged endothelial cells, and narrowed vessel lumen were observed in the muscle tissue. The findings of our study revealed that STZ-induced diabetes disrupted the vascular structure, while benfluorex partially improved it.

Keywords

benfluorexcapillarydiabetes mellitusstreptozotocin (STZ)transmission electron microscopyultrastructure
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 28 , Issue 6 , December 2022 , pp. 2167 - 2171
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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