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Ultrastructural Analysis of Healthy Synovial Fluids in Three Mammalian Species

Published online by Cambridge University Press:  18 March 2014

Constantin I. Matei ,
Caroline Boulocher ,
Christelle Boulé ,
Michael Schramme ,
Eric Viguier ,
Thierry Roger ,
Yves Berthier ,
Ana-Maria Trunfio-Sfarghiu  and
Marie-Geneviève Blanchin
Constantin I. Matei
Affiliation:
LaMCoS UMR5259, INSA-Lyon, CNRS, University of Lyon, 69621 Villeurbanne, France ILM, UMR5306-CNRS, University Claude Bernard Lyon 1,University of Lyon, 69622 Villeurbanne, France CTmu, University Claude Bernard Lyon 1, University of Lyon, 69622 Villeurbanne, France
Caroline Boulocher*
Affiliation:
UPSP ICE 2011-03-101, VetAgro Sup, Veterinary Campus, University Claude Bernard Lyon 1, University of Lyon, 69280 Marcy lʼEtoile, France
Christelle Boulé
Affiliation:
CTmu, University Claude Bernard Lyon 1, University of Lyon, 69622 Villeurbanne, France
Michael Schramme
Affiliation:
UPSP ICE 2011-03-101, VetAgro Sup, Veterinary Campus, University Claude Bernard Lyon 1, University of Lyon, 69280 Marcy lʼEtoile, France
Eric Viguier
Affiliation:
UPSP ICE 2011-03-101, VetAgro Sup, Veterinary Campus, University Claude Bernard Lyon 1, University of Lyon, 69280 Marcy lʼEtoile, France
Thierry Roger
Affiliation:
UPSP ICE 2011-03-101, VetAgro Sup, Veterinary Campus, University Claude Bernard Lyon 1, University of Lyon, 69280 Marcy lʼEtoile, France
Yves Berthier
Affiliation:
LaMCoS UMR5259, INSA-Lyon, CNRS, University of Lyon, 69621 Villeurbanne, France
Ana-Maria Trunfio-Sfarghiu
Affiliation:
LaMCoS UMR5259, INSA-Lyon, CNRS, University of Lyon, 69621 Villeurbanne, France
Marie-Geneviève Blanchin
Affiliation:
ILM, UMR5306-CNRS, University Claude Bernard Lyon 1,University of Lyon, 69622 Villeurbanne, France
*
*Corresponding author. [email protected]
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Abstract

A better knowledge of synovial fluid (SF) ultrastructure is required to further understand normal joint lubrication and metabolism. The aim of the present study was to elucidate SF structural features in healthy joints from three mammalian species of different size compared with features in biomimetic SF. High-resolution structural analysis was performed using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and environmental SEM/wet scanning transmission electron microscopy mode complemented by TEM and SEM cryogenic methods. Laser-scanning confocal microscopy (LCM) was used to locate the main components of SF with respect to its ultrastructural organization. The present study showed that the ultrastructure of healthy SF is built from a network of vesicles with a size range from 100 to a few hundred nanometers. A multilayered organization of the vesicle membranes was observed with a thickness of about 5 nm. LCM study of biological SF compared with synthetic SF showed that the microvesicles consist of a lipid-based membrane enveloping a glycoprotein gel. Thus, healthy SF has a discontinuous ultrastructure based on a complex network of microvesicles. This finding offers novel perspectives for the diagnosis and treatment of synovial joint diseases.

Keywords

synovial fluidultrastructuremicrovesicleslipid membraneslubrication
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 3 , June 2014 , pp. 903 - 911
Copyright
© Microscopy Society of America 2014 

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