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Time-Dependent Structural Changes in Porcine Stratum Corneum Following an Electroporating Pulse

Published online by Cambridge University Press:  02 July 2020

S.A. Gallo ,
A. Sen ,
M.L. Hensen ,
B. Chow  and
S.W. Hui
S.A. Gallo
Affiliation:
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, New York14263
A. Sen
Affiliation:
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, New York14263
M.L. Hensen
Affiliation:
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, New York14263
B. Chow
Affiliation:
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, New York14263
S.W. Hui
Affiliation:
Membrane Biophysics Laboratory, Roswell Park Cancer Institute, Buffalo, New York14263
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Extract

Electroporation has been used to permeabilize the skin to enhance the transdermal delivery of molecules. The mechanism is generally believed to be based on the temporary breakdown and permeabilization of the stratum corneum by the applied pulsed electric field. The kinetics of recovery are believed to be in the millisecond range. However, no structural changes within this time scale have been observed.

We studied the morphological changes to heat stripped porcine stratum corneum following an electroporating pulse by time-resolved freeze fracture electron microscopy. Pulses at a supraelectroporation threshold of 80 volts and 300 μsec were applied across the stratum corneum with a pair of copper plate electrodes, which also served as cooling contacts. The pulse timing was accomplished through the time-of-flight of a trigger bar attached to the plunging rod holding the sample.. The trigger bar passes through two optical switches. As the sample was propelled towards the liquid propane coolant container, the first switch triggered the pulse generator and the second marked the precise time at which the sample was frozen. The sample was pulsed at set times of 0.5 ms, 12ms, 5 sec, 60 sec, and 300 sec before freezing.

Type
Cryotechniques, Immunocytochemistry, and Electron Microscopy II. Cells and Tissues
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 442 - 443
Copyright
Copyright © Microscopy Society of America

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References

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5. This work is supported by Grant GM 55864 from the National Institutes of Health. Statistical analysis was performed with the help of Dr. William Greco, Biostatistics Facility, which is supported by the CCSR grant CA 16056 from the National Cancer Institute.Google Scholar