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Three-Dimensional Time-Lapse Digital Movie Analysis of the Developing Fruit Fly Eye in Organ Culture
Published online by Cambridge University Press: 02 July 2020
Extract
The compound eye of the fruit fly, Drosophila melanogaster, is composed of a highly ordered array of facets (FIG. 1), each containing a precise set of neurons and supporting cells. The eye arises during the third larval instar from an undifferentiated epithelium, the eye imaginai disc, which is connected to the brain via the optic stalk (FIG. 2). During eye development, movement of the morphogenetic furrow, progressive recruitment of specific cell types and the growth of photoreceptor axons into the brain are each dynamic processes that are routinely studied indirectly in fixed tissues. While stereotyped development and the ‘crystalline’ like structure of the eye facilitates this analysis, certain experiments are hindered by the inability to observe developmental processes as they occur. To overcome this limitation, we have combined organ culture with advanced microscopy tools to enable the observation of eye development in living tissue.
- Type
- Computational Advances and Enabling Technologies for 3D Microscopies in Biology
- Information
- Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 1129 - 1130
- Copyright
- Copyright © Microscopy Society of America 1997
References
1. Taylor, D.L.,et al., In “Current and Emerging Techniques in Monitoring Brain Structure and Function.” Annals of the New York Academy of Sciences, in press (1997).Google Scholar
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3. The authors thank Greg LaRocca, Robbin Debiasio, Mike Mantarro, Dave Pane, Chuck Nichols and Joseph Suhan for technical assistance.The work was supported by the NSF and undergraduate research grants from HHMI and CMU«SURG.Google Scholar