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Three-Dimensional Structure of Drosophila Testis Tip: The Spatial Relation Between Dividing Cells

Published online by Cambridge University Press:  06 August 2013

Kyung Eun Lee ,
Sung Sik Han  and
Hyesung Jeon
Kyung Eun Lee*
Affiliation:
Advanced Analysis Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-g, Seoul 136-791, Republic of Korea
Sung Sik Han
Affiliation:
School of Life Sciences and Biotechnology, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea
Hyesung Jeon
Affiliation:
Center for Theragnosis, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-g, Seoul 136-791, Republic of Korea
*
*Corresponding author. E-mail: [email protected]
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Abstract

At the apical tip of Drosophila testis, there is a stem cell niche known as the proliferation center, where the stem cells are maintained by hub cell cluster for the regulation of differentiation and proliferation. Germline stem cells go through mitosis four times from one primary spermatogonial cell to the 16-cell stage before the maturation. The cells derived from the same germline stem cell are located within one cyst, an enclosed system by two cyst cells, and they are connected by the intercellular bridges called ring canals. In this study, the three-dimensional (3D) structure of Drosophila testis tip was reconstructed from serial sections. The size of cells at each stage was compared in volume from the 3D structure. The stages of cells in a cyst could be distinguishable exactly by counting the cells linked with intercellular bridges in 3D-reconstructed structure. The cysts containing the same stage cells appeared in the horizontal plane. Both the germline stem cell directly attached to the hub cell and the spermatogonial cells detached from the hub cell were divided at the almost perpendicular direction to the spermatogonial cell layers. The dividing phase in one cyst was delayed gradually through the cytoplasmic region of intercellular bridge.

Keywords

3D reconstructionDrosophilatestisstem cell nichespermatogenesiscell division
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 188 - 193
Copyright
Copyright © Microscopy Society of America 2013 

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