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Three-Dimensional Reconstruction of Erythrocytes Using the Novel Method For Corrective Realignment of the Transmission Electron Microscopy Cross-Section Images

Published online by Cambridge University Press:  27 December 2018

Yuzhou Fan ,
Djordje Antonijević Open the ORCID record for Djordje Antonijević [Opens in a new window] ,
Xing Zhong ,
Vladimir S. Komlev ,
Zhiyu Li ,
Marija Đurić  and
Yifang Fan
Yuzhou Fan
Affiliation:
School of Physical Education and Sport Science, Fujian Normal University, 350108 Fuzhou, China Shenzhen Tourism College, Jinan University, 518053 Shenzhen, China
Djordje Antonijević
Affiliation:
School of Physical Education and Sport Science, Fujian Normal University, 350108 Fuzhou, China Laboratory for Anthropology, Institute for Anatomy, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia Laboratory for Atomic Physics, Institute for nuclear science “Vinca”, University of Belgrade, 11000 Belgrade, Serbia
Xing Zhong
Affiliation:
Medical Imaging Center, The First Affiliated Hospital of Jinan University, 510632 Guangzhou, China
Vladimir S. Komlev
Affiliation:
A. A. Baikov Institute of Metallurgy and Materials Science, 119334 Moscow, Russia
Zhiyu Li
Affiliation:
College of Foreign Studies, Jinan University, 510632 Guangzhou, China
Marija Đurić
Affiliation:
Laboratory for Anthropology, Institute for Anatomy, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia
Yifang Fan*
Affiliation:
School of Physical Education and Sport Science, Fujian Normal University, 350108 Fuzhou, China
*
*Author for correspondence: Yifang Fan, E-mail: [email protected]
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Abstract

The detailed kinetics study of erythrocyte deformability is useful for the early diagnosis of blood diseases and for monitoring the blood rheology. Present solutions for a three-dimensional (3D) reconstruction of erythrocytes has a limited potential. This study aimed to use erythrocyte transmission electron images (ETIs) to evaluate the morphological relationship between adjacent ETIs and generate erythrocytes 3D model. First, ultrathin serial sections of skeletal muscle tissue were obtained using an ultramicrotome. Further, the set of ETIs in a capillary were captured by transmission electron microscopy. The images were aligned by translations and rotations using custom software to optimize the morphological relationship between adjacent ETIs. These coordinate transformations exploit the unique principal axis of inertia of each image to define the body coordinate system and hence provide the means to accurately reconnect the adjacent ETIs. The sum of the distances between the corresponding points on the boundary of adjacent ETIs was minimized and, further, was optimized by using physiological relationship between the adjacent ETIs. The analysis allowed to define precise virtual relationship between the adjacent erythrocytes. Finally, extracted erythrocytes’ cross-section images allowed to generate 3D model of the erythrocytes.

Keywords

erythrocytethree-dimensional reconstructiontransmission electron microscopymicroanalysis
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 24 , Issue 6 , December 2018 , pp. 676 - 683
Copyright
© Microscopy Society of America 2018 

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Footnotes

a

The authors equally contributed to the manuscript and share the first authorship.

Cite this article: Fan Y, Antonijević D, Zhong X, Komlev VS, Li Z, Đurić M and Fan Y (2018) Three-Dimensional Reconstruction of Erythrocytes Using the Novel Method for Corrective Realignment of the Transmission Electron Microscopy Cross-Section Images. Microsc Microanal24(6), 676–683. doi: 10.1017/S1431927618015325

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