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Three- Dmensi0nal(3d) Relationships Between Tumor Cell Proliferation and its Anigiogensis in Human Breast Cancer by Confocal Laser Scanning Miroscopy(CLSM) with Triple-Labeling Immunohistochemistry.

Published online by Cambridge University Press:  02 July 2020

J. Itoh ,
K. Yasumura ,
K. Ogawa ,
K. Kawai ,
A. Serizawa  and
R. Y. Osamura
J. Itoh
Affiliation:
Lab for Struct/Funct Res, 259-11, Japan
K. Yasumura
Affiliation:
Dept of Surgery210Japan
K. Ogawa
Affiliation:
Dept of Diag Radiol NKK Hospital Kawasaki, 210Japan
K. Kawai
Affiliation:
Div of Diag Pathol259-11Japan
A. Serizawa
Affiliation:
Div of Diag Pathol259-11Japan
R. Y. Osamura
Affiliation:
Dept of Pathol Tokai Univ Sch of Med Isehara259-11Japan
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Extract

There have been considerable interests in the relationship between tumor cell proliferation and angiogenesis. In human breast carcinomas, it has been reported that angiogenesis is an independent prognostic factor and that proliferating factors are closely related with lymph node status. Therefore, the demonstration of proliferating and angiogenetic factors as well as the tumor blood vessels in human breast carcinoma simultaneously is important for clinical evaluation of breast cancer patients.

CLSM has been an exciting new apparatus because of it's more powerful resolution than conventional light microscopy and the high-performance on 3D optical sectioning. Images taken with CLSM can be digitized and made variable for image analysis manipulation and computer-assisted 3D reconstructions.

In the present study, we employed immunohistochemical comparative 3D visualization of proliferating and angiogenstic factors with microvessels in human breast carcinomas by CLSM.

The tissues from the surgically resected breast carcinoma were fixed in cold 4 % paraformaldehyde(PFA) and routinely processed in paraffin wax.

Type
Neoplasia: Abnormal Cell Growth Or Death/Apoptosis? Insights From Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 29 - 30
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Itoh, J., et al.,(1992), J. Histochem. Çytochem., 40:955–96710.1177/40.7.1607644CrossRefGoogle Scholar
2.Itoh, J., et al.,MRT(in press)Google Scholar