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The Technical Aspects for Studying Morphology of Purified Lacrimal Gland Acinar Cells Cultured on Matrigel Rafts: A New Model System for Studying Lacrimal Physiology

Published online by Cambridge University Press:  02 July 2020

Mike Pidgeon ,
Joel Schechter, Ph.D ,
Natalie Chang, M.S. ,
Donald Chang ,
Melvin Trousdale, Ph.D.  and
Doug Stevenson
Mike Pidgeon
Affiliation:
Dept. of Cell and Neurobiology, University of Southern California, Keck School of Medicine, 1333 San Pablo St., Los angeles, CA90089-9112
Joel Schechter, Ph.D
Affiliation:
Dept. of Cell and Neurobiology, University of Southern California, Keck School of Medicine, 1333 San Pablo St., Los angeles, CA90089-9112
Natalie Chang, M.S.
Affiliation:
Dept. of Cell and Neurobiology, University of Southern California, Keck School of Medicine, 1333 San Pablo St., Los angeles, CA90089-9112
Donald Chang
Affiliation:
Dept. of Cell and Neurobiology, University of Southern California, Keck School of Medicine, 1333 San Pablo St., Los angeles, CA90089-9112
Melvin Trousdale, Ph.D.
Affiliation:
Dept. of Opthamology, University of Southern California, Keck School of Medicine, Los Angeles, CA90089-9112
Doug Stevenson
Affiliation:
Dept. of Opthamology, University of Southern California, Keck School of Medicine, Los Angeles, CA90089-9112
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Extract

Objective: To demonstrate the quality of a new model which gives a close representation of the in situ gland for studying functional characteristics of lacrimal acinar cells. A method has been long sought which would serve as a good in-situ model for studying functional attributes of lacrimal acinar cells. Previous methods used cell plates coated with Matrigel as a growth interface for lacrimal acinar cells.

Two problems associated with this method are: cell cultures seeded this way provided a restrictive, planar growth interface, and the inability to maintain cultures beyond seven days, due in part to a lack of proliferation. A modification of recent methods (Guo et al., 1998) was implemented. The cell preparations were coated with a Hepato Stim® Medium (supplemented with EGF, DHT) and Matrigel. The medium is an extracellular matrix enriched solubilized preparation developed from the basement membranes of the Englebreth-Holm-Swarm mouse sarcoma line.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 894 - 895
Copyright
Copyright © Microscopy Society of America

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References

1. Guo, Z., Azzarolo, A.M., Mircheff, A.K., Schechter, J., Warren, D.W., Wood, R.L., and Kaslow, H.R.. 1998 Induction of apparent autoimmune dacryoadenitis in rabbits. ARVO Annual Meeting.Google Scholar