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Studies on the Green Fluorescence Protein Marked Autographa Californica Multiple Nucleopolyhedrovirus in Trichoplusia ni Larvae.

Published online by Cambridge University Press:  02 July 2020

A.J. Brownwright ,
J.W. Barrett  and
S.R. Palli
A.J. Brownwright
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, P.O.Box 490, Sault Ste.Marie, Ontario, CanadaP6A 5M7
J.W. Barrett
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, P.O.Box 490, Sault Ste.Marie, Ontario, CanadaP6A 5M7
S.R. Palli
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, P.O.Box 490, Sault Ste.Marie, Ontario, CanadaP6A 5M7
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Extract

We have investigated the infection process of Green Fluorescence Protein marked Autographa Californica multiple nucleopolyhedrovirusCAcMNPV-GFP) under both light and electron microscopes. Enveloped virus particles were observed in the nucleus of columnar epithelial cells of the midgut 24 hours after feeding Trichoplusia ni larvae with AcMNPV-GFP infected freeze-dried larvae. by this time enveloped virus particles were also observed in the plasma membrane reticular system(PMRS) of midgut epithelial cells and nuclei of the tracheoblasts that are present in the connective tissue of the midgut. by 60 hours post feeding, tissues such as fat body and epidermis were showing enveloped virus particles in their nuclei while the the midgut epithelial cells were now largely devoid of virus.

Based on these results we propose that enveloped virions released from occlusion bodies in the lumen enter columnar epithelial cells and replicate in the nucleus. At the same time some of the virions enter the PMRS and get quick access to tracheoblasts present in the midgut connective tissue and thereafter to hemocytes present in the haemolymph.

Type
Cytochemistry, Histochemistry, Immunocytochemistry, and in Situ Hybridization
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 169 - 170
Copyright
Copyright © Microscopy Society of America 1997

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