Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-23T16:41:13.160Z Has data issue: false hasContentIssue false

Structural Studies of Dicer-2 Complexes

Published online by Cambridge University Press:  22 July 2022

Helen Donelick
Affiliation:
Department of Biochemistry, University of Utah, Salt Lake City, UT, United States
Brenda Bass*
Affiliation:
Department of Biochemistry, University of Utah, Salt Lake City, UT, United States
Peter Shen*
Affiliation:
Department of Biochemistry, University of Utah, Salt Lake City, UT, United States
*
*Co-corresponding authors: [email protected] and [email protected]
*Co-corresponding authors: [email protected] and [email protected]

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Copyright
Copyright © Microscopy Society of America 2022

References

Welker, N. C. et al. , “Dicer's helicase domain discriminates dsRNA termini to promote an altered reaction mode,” Mol. Cell, vol. 41, no. 5, pp. 589599, 2011, doi: 10.1016/j.molcel.2011.02.005.CrossRefGoogle ScholarPubMed
Sinha, N. K., Trettin, K. D., Aruscavage, P. J., and Bass, B. L., “Drosophila Dicer-2 cleavage is mediated by helicase- and dsRNA termini-dependent states that are modulated by loquacious-PD,Mol. Cell, vol. 58, no. 3, pp. 406417, 2015, doi: 10.1016/j.molcel.2015.03.012.CrossRefGoogle Scholar
Sinha, N. K., Iwasa, J., Shen, P. S., and Bass, B. L., “Dicer uses distinct modules for recognizing dsRNA termini,Science (80-.)., vol. 359, no. 6373, pp. 329334, 2018, doi: 10.1126/science.aaq0921.CrossRefGoogle Scholar
Nishida, K. M., Miyoshi, K., Ogino, A., Miyoshi, T., Siomi, H., and Siomi, M. C., “Roles of R2D2, a Cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila,” Mol. Cell, vol. 49, no. 4, pp. 680691, 2013, doi: 10.1016/j.molcel.2012.12.024.CrossRefGoogle ScholarPubMed
Liu, Q. et al. , “R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway,” Science (80-.)., vol. 301, no. 5641, pp. 19211925, 2003, doi: 10.1126/science.1088710.CrossRefGoogle ScholarPubMed
Liu, X., Jiang, F., Kalidas, S., Smith, D., and Liu, Q., “Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes,” Rna, vol. 12, no. 8, pp. 15141520, 2006, doi: 10.1261/rna.101606.CrossRefGoogle ScholarPubMed
Liu, Z. et al. , “Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate,” Cell, vol. 173, no. 5, pp. 11911203, 2018, doi: 10.1016/j.cell.2018.03.080.CrossRefGoogle ScholarPubMed
Jonely, M., Singh, R. K., Donelick, H. M., Bass, B. L., and Noriega, R., “Loquacious-PD regulates the terminus-dependent molecular recognition of Dicer-2 toward double-stranded RNA,” Chem. Commun., vol. 57, no. 83, pp. 1087910882, 2021, doi: 10.1039/d1cc03843e.CrossRefGoogle ScholarPubMed