Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T11:38:03.123Z Has data issue: false hasContentIssue false
  • English
  • Français

Structural determination of the Dicer-2•R2D2 complex

Published online by Cambridge University Press:  30 July 2021

Helen Donelick ,
Peter Shen  and
Brenda Bass
Helen Donelick
Affiliation:
University of Utah, Salt Lake City, Utah, United States
Peter Shen
Affiliation:
University of Utah, Salt Lake City, Utah, United States
Brenda Bass
Affiliation:
University of Utah, Salt Lake City, Utah, United States

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1122 - 1124
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Elbashir, S. M., Lendeckel, W. & Tuschl, T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15, 188–200 (2001).CrossRefGoogle ScholarPubMed
Nishida, K. M. et al. Roles of R2D2, a Cytoplasmic D2 Body Component, in the Endogenous siRNA Pathway in Drosophila. Mol. Cell 49, 680–691 (2013).Google ScholarPubMed
Liu, Q. et al. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science (80-. ). 301, 1921–1925 (2003).CrossRefGoogle ScholarPubMed
Liu, X., Jiang, F., Kalidas, S., Smith, D. & Liu, Q. Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes. Rna 12, 1514–1520 (2006).CrossRefGoogle ScholarPubMed
Hansen, S. R., Aderounmu, A. M., Donelick, H. M. & Bass, B. L. Dicer's Helicase Domain: A Meeting Place for Regulatory Proteins. Cold Spring Harb. Symp. Quant. Biol. 84, 039750 (2020).Google Scholar
Sinha, N. K., Iwasa, J., Shen, P. S. & Bass, B. L. Dicer uses distinct modules for recognizing dsRNA termini. Science (80-. ). 359, 329–334 (2018).CrossRefGoogle ScholarPubMed
Bepler, T. et al. Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nat. Methods (2019) doi:10.1038/s41592-019-0575-8.CrossRefGoogle ScholarPubMed
Bepler, T., Kelley, K., Noble, A. J. & Berger, B. Topaz-Denoise: general deep denoising models for cryoEM and cryoET. Nat. Commun. (2020) doi:10.1038/s41467-020-18952-1.CrossRefGoogle ScholarPubMed