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Strategies for Data Flow and Storage for High Throughput, High Resolution Cryo-EM Data Collection

Published online by Cambridge University Press:  05 August 2019

William J. Rice ,
Anchi Cheng ,
Sargis Dallakyan ,
Swapnil Bhatkar ,
Shaker Krit ,
Edward T. Eng ,
Bridget Carragher  and
Clinton S. Potter
William J. Rice*
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA. National Center for Cryo-EM Access and Training, New York Structural Biology Center, New York, NY, USA.
Anchi Cheng
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA. National Center for Cryo-EM Access and Training, New York Structural Biology Center, New York, NY, USA.
Sargis Dallakyan
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Swapnil Bhatkar
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Shaker Krit
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Edward T. Eng
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA. National Center for Cryo-EM Access and Training, New York Structural Biology Center, New York, NY, USA.
Bridget Carragher
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA. National Center for Cryo-EM Access and Training, New York Structural Biology Center, New York, NY, USA. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Clinton S. Potter
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA. National Center for Cryo-EM Access and Training, New York Structural Biology Center, New York, NY, USA. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
*
*Corresponding author: [email protected]

Abstract

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Type
From Images to Insights: Working with Large Data in Cell Biological Imaging
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1394 - 1395
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Cheng, Y, Science 361(6405) (2018), p. 876.Google Scholar
[2]Lander, GC et al. , J Struct Biol 166(1) (2009), p. 95.Google Scholar
[3]Scheres, SH, J Struct Biol 180(3) (2012), p. 519.Google Scholar
[4]Zheng, SQ et al. , Nat Methods 14(4) (2017), p. 331.Google Scholar
[5]Eng, ET et al. , bioRxiv (2018).Google Scholar
[6]This work was supported by grants from the Simons Foundation (349247), NYSTAR, and the NIH National Institute of General Medical Sciences (GM103310) with additional support from the Agouron Institute [Grant Number: F00316] and NIH S10 OD019994-01.Google Scholar