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The Stem Vasculature of Na1/Na1 and Na2/Na2 Mutants in Maize

Published online by Cambridge University Press:  02 July 2020

Wayne Y. Cheng ,
Ping-chin Cheng ,
Miṅ Gu ,
Xiaosong Gan  and
David B. Walden
Wayne Y. Cheng
Affiliation:
Williamsville East High School, Williamsville, New York , 14051 , USA
Ping-chin Cheng
Affiliation:
Dept. of Electrical Engineering, University of Buffalo, NY , 14260 , USA
Miṅ Gu
Affiliation:
Centre for Nano-Photonics, Swinburne of Technology, Melbourne, Australia
Xiaosong Gan
Affiliation:
Centre for Nano-Photonics, Swinburne of Technology, Melbourne, Australia
David B. Walden
Affiliation:
Dept. of Plant Sciences, Univ. of Western Ontario, London, Ontario, CanadaN6A 5B7
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Abstract

The vasculature of two recessive dwarf mutants, nana-1 (nal) (FIG 1) and nana-2 (na2) (FIG 2) of maize (Zea mays)were studied. All the plants used in this study were grown at the field station of University of Western Ontario, London, Ontario, Canada, in the summer of 2000. The specimens were fixed in methanol, serially sectioned with a razor blade using a specially made jig. The image was obtained by using a modified Acer 620T flat-bed scanner in liquid (Cheng et al., 2001). The optical sections were obtained by twophoton florescence microscopy using an Olympus Fluorview FL300 confocal microscope equipped with a Spectra-Physics Mai-Tai tunable ultra-fast Ti-sapphire laser. Excitation wavelengths was set at 780nm NIR with a pulse width of l00fs, <550nm autofluorescence was detected. Extended focus views were generated by using AutoQuant's AutoVisualize-3D™ software.

Different from the parallel arranged longitudinal vascular bundles commonly found in the internodes of wild type, nal/nalhas a complex stem vasculature (FIG 3)(Cheng et al., 2000a; 2000b).

Type
Student Research Forum (Organized by R. Koch and Z. Mason)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 444 - 445
Copyright
Copyright © Microscopy Society of America 2001

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References

Cheng, W Y et al., Microscopy and Microanalysis, (2001) (This Volume).Google Scholar
Cheng, W Y et al., Microscopy and Microanalysis, 6, Supp. 2 (2000a) 692–693.CrossRefGoogle Scholar
Cheng, W Y et al., Scanning, 22 (2000b) 189–190.Google Scholar