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Soma/Germline Interactions In Caenorhabditis Elegans Gonad

Published online by Cambridge University Press:  02 July 2020

David H. Hall ,
Virginia P. Winfrey ,
Gareth Blaeuer ,
Loren H. Hoffman ,
Tokiko Furuta ,
Kimberly L. Rose ,
Oliver Hobert  and
David Greenstein
David H. Hall
Affiliation:
Center for C. elegans Anatomy, Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway, Bronx, NY10461
Virginia P. Winfrey
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
Gareth Blaeuer
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
Loren H. Hoffman
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
Tokiko Furuta
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
Kimberly L. Rose
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
Oliver Hobert
Affiliation:
Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, NY10032
David Greenstein
Affiliation:
Department of Cell Biology, Vanderbilt University School of Medicine, 21st and Garland, Nashville, TN37232
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Extract

A combination of microscopical methods (TEM, SEM, freeze fracture, confocal, immunoEM) are utilized to demonstrate functional interactions between somatic cells and the germline in the model organism, C. elegans.A variety of signaling mechanisms are required to coordinate activity of maturing oocytes and the somatic sheath of the gonad. Communication via gap junctions allows direct coordination of sheath cell motility and oocyte development. Gap junctions link adjoining sheath cells, and link the sheath to the most mature oocyte. Yolk protein produced in the intestine passes from the pseudocoelom through the basal lamina of the proximal gonad and then through fenestrations (sheath pores) in the gonad sheath before being endocytosed into the most mature oocytes. Myoepithelial specializations anchored by hemi-adherens junctions in the proximal sheath act to squeeze the primary oocyte out of the gonad to the spermatheca for fertilization.

Type
Applications of Imaging Techniques to the Study of Embryological Development
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1072 - 1073
Copyright
Copyright © Microscopy Society of America

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References

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