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Soft Microscopy of Negative Stained Soft Materials: Balancing Dose Rate and Sample Damage

Published online by Cambridge University Press:  30 July 2021

Chamille Lescott ,
Roberto dos Reis ,
Mallika Modak ,
Evan Scott  and
Vinayak Dravid
Chamille Lescott
Affiliation:
Department of Materials Science & Engineering, Northwestern University, United States
Roberto dos Reis
Affiliation:
Department of Materials Science and Engineering, Northwestern University, United States
Mallika Modak
Affiliation:
Department of Biomedical Engineering, Northwestern University, United States
Evan Scott
Affiliation:
Department of Biomedical Engineering, Northwestern University, United States
Vinayak Dravid
Affiliation:
Department of Materials Science and Engineering, Northwestern University, United States

Abstract

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Type
To Fix or Not To Fix? A Question for Biological Samples
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1408 - 1411
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

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This work was primarily supported by Air Force Office of Scientific Research (AFSOR) FA9550-17-1-0348 (subproject for Reconfigurable Matter from Programmable Atom Equivalents) and C-ABN(AFRL) FA8650-15-2-5518 (Statistics and Dynamics of Soft Biological Structures and Hybrid Soft-Hard Assemblies). Additional funding from ARO/MURI W911NF1810200 (Dravid Subproject for Stimuli-Responsive Control of Protein-Based Molecular Biology). The research of Evan A Scott is supported by the National Science Foundation (CAREER-1453576), the National Institutes of Health Director's New Innovator Award (NHLBI 1DP2HL132390-01), and the National Institute of Allergy and Infectious Disease (NIAID 5R01AI145345-02). This work made use of the BioCryo facility of Northwestern University's NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern's MRSEC program (NSF DMR-1720139).Google Scholar