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Small Molecule Microcrystal Electron Diffraction (MicroED) for the Pharmaceutical Industry – Results from Examining Over Fifty Samples

Published online by Cambridge University Press:  30 July 2021

Jessica Bruhn
Affiliation:
NanoImaging Services, San Diego, California, United States
Giovanna Scapin
Affiliation:
Nanoimaging Services, New York, New York, United States
Anchi Cheng
Affiliation:
NanoImaging Services, San Diego, California, United States
Thejusvi Ganesh
Affiliation:
NanoImaging Services, San Diego, California, United States
Sargis Dallakyan
Affiliation:
NanoImaging Services, San Diego, California, United States
Brandon Read
Affiliation:
NanoImaging Services, San Diego, California, United States
Travis Nieusma
Affiliation:
NanoImaging Services, United States
Kyle Lucier
Affiliation:
NanoImaging Services, United States
Megan Mayer
Affiliation:
NanoImaging Services, United States
Nicole Chiang
Affiliation:
NanoImaging Services, United States
Nicole Poweleit
Affiliation:
NanoImaging Services, United States
Philip McGilvray
Affiliation:
NanoImaging Services, United States
Timothy Wilson
Affiliation:
NanoImaging Services, United States
Michael Mashore
Affiliation:
NanoImaging Services, United States
Camille Hennessy
Affiliation:
NanoImaging Services, United States
Sean Thomson
Affiliation:
NanoImaging Services, United States
Clinton Potter
Affiliation:
NanoImaging Services, United States
Bridget Carragher
Affiliation:
New York Structural Biology Center, New York, New York, United States

Abstract

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Type
Cryo-EM in Drug Discovery
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Gruene, T., Wennmacher, J.T.C., Zaubitzer, C., Holstein, J.J., Heidler, J., Fecteau-Lefebvre, A., De Carlo, S., Muller, E., Goldie, K.N., Regeni, I., Li, T., Santiso-Quinones, G., Steinfeld, G., Handschin, S., van Genderen, E., van Bokhoven, J.A., Clever, G.H., and Pantelic, R.. 2018. Rapid Structure Determination of Microcrystalline Molecular Compounds Using Electron Diffraction. Angew Chem Int Ed Engl. 57:16313-16317.CrossRefGoogle ScholarPubMed
Jones, C.G., Martynowycz, M.W., Hattne, J., Fulton, T.J., Stoltz, B.M., Rodriguez, J.A., Nelson, H.M., and Gonen, T.. 2018. The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination. ACS Central Science. 4:1587-1592.CrossRefGoogle ScholarPubMed
Cheng, A., Negro, C., Bruhn, J.F., Rice, W.J., Dallakyan, S., Eng, E.T., Waterman, D.G., Potter, C.S., and Carragher, B.. 2021. Leginon: New features and applications. Protein Sci. 30:136-150.CrossRefGoogle ScholarPubMed
Clabbers, M.T.B., Gruene, T., Parkhurst, J.M., Abrahams, J.P., and Waterman, D.G.. 2018. Electron diffraction data processing with DIALS. Acta Crystallographica Section D: Structural Biology. 74:506-518.CrossRefGoogle ScholarPubMed
Sheldrick, G.M. 2015b. SHELXT - integrated space-group and crystal-structure determination. Acta Crystallogr A Found Adv. 71:3-8.CrossRefGoogle Scholar
Schneider, T.R., and Sheldrick, G.M.. 2002. Substructure solution with SHELXD. Acta Crystallogr D Biol Crystallogr. 58:1772-1779.CrossRefGoogle ScholarPubMed
McCoy, A.J., Grosse-Kunstleve, R.W., Adams, P.D., Winn, M.D., Storoni, L.C., and Read, R.J.. 2007. Phaser crystallographic software. J Appl Crystallogr. 40:658-674.Google ScholarPubMed