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Single-Molecule Localization Super-Resolution Microscopy: Deeper and Faster

Published online by Cambridge University Press:  31 October 2012

Sébastien Herbert ,
Helena Soares ,
Christophe Zimmer  and
Ricardo Henriques
Sébastien Herbert
Affiliation:
Institut Pasteur, Groupe Imagerie et Modélisation, CNRS URA 2582, 25 rue du Docteur Roux, 75015 Paris, France Frontiers in Life Sciences PhD Program, University Paris Diderot, 5 rue Thomas-Mann, 75013 Paris, France
Helena Soares
Affiliation:
Institut Pasteur, Lymphocyte Cell Biology Unit, CNRS URA 1961, 28 rue du Docteur Roux, 75015 Paris, France
Christophe Zimmer*
Affiliation:
Institut Pasteur, Groupe Imagerie et Modélisation, CNRS URA 2582, 25 rue du Docteur Roux, 75015 Paris, France
Ricardo Henriques*
Affiliation:
Institut Pasteur, Groupe Imagerie et Modélisation, CNRS URA 2582, 25 rue du Docteur Roux, 75015 Paris, France
*
*Corresponding author: E-mail: [email protected]
**Corresponding author: E-mail: [email protected]
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Abstract

For over a decade fluorescence microscopy has demonstrated the capacity to achieve single-molecule localization accuracies of a few nanometers, well below the ∼200 nm lateral and ∼500 nm axial resolution limit of conventional microscopy. Yet, only the recent development of new fluorescence labeling modalities, the increase in sensitivity of imaging hardware, and the creation of novel image analysis tools allow for the emergence of single-molecule-based super-resolution imaging techniques. Novel methods such as photoactivated localization microscopy and stochastic optical reconstruction microscopy can typically reach a tenfold increase in resolution compared to standard microscopy methods. Their implementation is relatively easy only requiring minimal changes to a conventional wide-field or total internal reflection fluorescence microscope. The recent translation of these two methods into commercial imaging systems has made them further accessible to researchers in biology. However, these methods are still evolving rapidly toward imaging live samples with high temporal resolution and depth. In this review, we recall the roots of single-molecule localization microscopy, summarize major recent developments, and offer perspective on potential applications.

Keywords

single moleculesuper-resolutionfluorescencemicroscopyPALMSTORM
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 6 , December 2012 , pp. 1419 - 1429
Copyright
Copyright © Microscopy Society of America 2012

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