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Simultaneous Localization and Quantitation of Progesterone Receptor Immunoreactivity in Human Breast Carcinomas.

Published online by Cambridge University Press:  02 July 2020

K.A. Roth
K.A. Roth*
Affiliation:
Departments of Pathology and Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO, 63110
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Extract

The presence of estrogen and progesterone receptors on human breast carcinomas correlates with both response to hormonal therapy and survival. Quantitation of receptor activity is typically performed by radioreceptor assay on fresh frozen tumor tissue obtained at the time of surgical resection. Estrogen and progesterone receptor reactivity can also be detected immunohistochemically on frozen sections of breast cancer and in appropriately fixed paraffin embedded tissues. Several studies have shown that semi-quantitative visual determination and quantitative image analysis assessment of estrogen and progesterone receptor immunoreactivity on fixed tissue sections is highly correlated with biochemical measurements of receptor in frozen samples. We recently developed a procedure for the simultaneous localization and quantitation of antigens in fixed tissue samples that does not involve antigen extraction, radioactive materials, or image analysis. In this procedure, which we term a “Midwestern assay”, fixed tissue sections are used with antigen-specific enzyme-linked antibodies to generate soluble reaction products which are spectrophotometrically quantitatable, as in an ELISA, and deposited reaction products which are microscopically localizable, as in enzyme-based immunohistochemistry.

Type
Neoplasia: Abnormal Cell Growth or Death/Apoptosis? Insights from Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 7 - 8
Copyright
Copyright © Microscopy Society of America 1997

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References

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6. This work was supported by funds from the Washington University School of Medicine, Department of Pathology. I acknowledge the expert assistance received from the Washington University School of Medicine, Department of Molecular Biology and Pharmacology Histology facility.Google Scholar