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A Simple Method for the Cross-Section Area Determination of Single Profiled Fibers and Its Application

Published online by Cambridge University Press:  30 January 2018

Zhangchuan Peng ,
Chun Liu ,
Lin Zhang ,
Wei Li ,
Wenbo Hu ,
Sanyuan Ma  and
Qingyou Xia
Zhangchuan Peng
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Chun Liu*
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China
Lin Zhang
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Wei Li
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Wenbo Hu
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Sanyuan Ma
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Qingyou Xia
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China
*
Author for correspondence: Chun Liu, E-mail: [email protected]
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Abstract

One of the critical prerequisites for accurately measuring the mechanical properties of profiled fibers is the precise determination of their cross-sectional areas (CSAs). In this study, a new method is established for determining a single profiled fibers’ CSA based on the frozen section method and digital photo, pixel-ratio method (FS-DP). FS-DP is used to obtain a transverse section of a fiber, by acquiring an image of the cross section using optical microscopy or scanning electron microscopy, and then calculating the CSA using Photoshop. Using FS-DP, it was found that the shape of a fiber of silk changes little in a range of 50 μm, but varies considerably over a range of 1 m, while the CSA of cocoon silk (900 m) first increases and then decreases. Mechanical property tests showed that the elongation, strength, elastic modulus, and toughness values of the cocoon silk are consistent with those reported previously. Additionally, FS-DP was also used to observe other profiled fibers. The application tests indicated that FS-DP can be used to quickly and accurately obtain the CSA of a single profiled fiber, and that it is suitable for the large-scale determination and analysis of the mechanical properties of profiled fibers.

Keywords

frozen section methodcross-sectional areaprofiled fibersilkworm silk fiberoptical microscope
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 24 , Issue 1 , February 2018 , pp. 17 - 28
Copyright
© Microscopy Society of America 2018 

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References

ASTM (2004) Standard Test Method for Tensile Strength and Young’s Modulus of Fibers. West Conshohocken, PA: ASTM International.Google Scholar
ASTM (2009) Standard Test Method for Resistance to Airflow as an Indication of Average Fiber Diameter of Wool Top, Card Sliver, and Scoured Wool. West Conshohocken, PA: ASTM International.Google Scholar
ASTM (2010) Standard Test Method for Diameter of Wool and Other Animal Fibers By Sirolan-Laserscan Fiber Diameter Analyser. West Conshohocken, PA: ASTM International.Google Scholar
ASTM (2013) Standard Test Method for Diameter of Wool and Other Animal Fibers by Microprojection. West Conshohocken, PA: ASTM International.Google Scholar
Baviskar, SN (2011) A quick & automated method for measuring cell area using ImageJ. Am Biol Teacher 73(9), 554–556.CrossRefGoogle Scholar
Bradshaw, JD, Rice, ME and Hill, JH (2007) Digital analysis of leaf surface area: Effects of shape, resolution, and size. J Kansas Entomol Soc 80(4), 339–347.CrossRefGoogle Scholar
Cao, Y, Wu, Y and Goda, K (2008) Research and development of bast fiber-reinforced composites. Cailiao Yanjiu Xuebao/Chin J Mater Res 22(1), 10–17.Google Scholar
Cheung, HY, Lau, KT, Ho, MP and Mosallam, A (2009) Study on the mechanical properties of different silkworm silk fibers. J Compos Mater 43(43), 2521–2531.CrossRefGoogle Scholar
Gao, JC, Guo, GJ, Guo, YM, Wang, XX and Yong-Chen, DU (2011) Measuring plant leaf area by Scanner and ImageJ software. China Vegetables 2, 73–77.Google Scholar
Gosline, J, Guerette, P, Ortlepp, C and Savage, K (1999) The mechanical design of spider silks: From fibroin sequence to mechanical function. J Exp Biol 202(23), 3295–3303.CrossRefGoogle ScholarPubMed
Ishtiaque, SM, Sen, K and Kumar, A (2013) Study of engineered carpet yarns structure; Part B: By cross-sectional microtomy. J Ind Text 44(4), 625–638.Google Scholar
JeoleX (2016) Estimation of leaf area by Pixel Counting Method in Photoshop CS6. Available at http://www.instructables.com/id/Estimation-of-Leaf-Area-by-Pixel-Counting-Method-i/: Instructables (retrieved February 13, 2016).Google Scholar
Jian, N (1997) An instrument for precise measurement of cross-sectional area and Young’s modulus of single fiber by resonance method with contactless detection system. J SYSU (Natural Science Edition) 36(3), 119–121.Google Scholar
Jiang, P, Liu, H, Wang, C, Wu, L, Huang, J and Guo, C (2006) Tensile behavior and morphology of differently degummed silkworm (Bombyx mori) cocoon silk fibres. Mater Lett 60(7), 919–925.CrossRefGoogle Scholar
Kawahara, Y, Shioya, M and Takaku, A (1996) Mechanical properties of silk fibers treated with methacrylamide [J]. J Appl Polym Sci 59(51), 1359–1364.3.0.CO;2-U>CrossRefGoogle Scholar
Kuwana, Y, Sezutsu, H, Nakajima, K-I, Tamada, Y and Kojima, K (2014) High-toughness silk produced by a transgenic silkworm expressing spider (Araneus ventricosus) dragline silk protein. PLoS One 9(8), e105325.CrossRefGoogle ScholarPubMed
Lewis, M (2014) APES treatment of slides. Available at http://theolb.readthedocs.io/en/latest/molecular-biology/apes-treatment-of-slides.html. The Open Lab Book.Google Scholar
Liu, F (2014) The influence factors of metal materials tensile test at room temperature (in Chinese). Sci Technol Enterprise 15(10), 286–286.Google Scholar
Liu, X and Wang, F (2011) Applications of non-circular cross-section chemical fibers. Chem Fibers Int 61(4), 210–212.Google Scholar
Mortimer, B, Holland, C and Vollrath, F (2013) Forced reeling of Bombyx mori silk: Separating behavior and processing conditions. Biomacromolecules 14(10), 3653–3659.CrossRefGoogle ScholarPubMed
Nakamae, K, Nishino, T and Ohkubo, H (1989) Elastic modulus of the crystalline regions of silk fibroin. Polymer 30(7), 1243–1246.CrossRefGoogle Scholar
Neckář, B, Ishtiaque, SM, Švehlová, L, Neckář, B and Švehlová, L (1988) Rotor yarn structure by cross-sectional microtomy. Text Res J 58(11), 625–632.Google Scholar
Omenetto, FG and Kaplan, DL (2010) New opportunities for an ancient material. Science 329(5991), 528–531.CrossRefGoogle ScholarPubMed
Pérez-Rigueiro, J, Viney, C, Llorca, J and Elices, M (1998) Silkworm silk as an engineering material. J Appl Poly Sci 70(12), 2439–2447.3.0.CO;2-J>CrossRefGoogle Scholar
Petkar, B, Oka, P and Sundaram, V (1980) The cross-sectional shapes of a cotton fiber along its length. Text Res J 50(9), 541–543.CrossRefGoogle Scholar
Qin, H, Xilong, MA, Chuanshun, HU, Zhou, B and Cui, Y (2013) Ferrite percentage measurement of Duplex stainless steel layering based on counting and Photoshop pixel method. J Liaoning Shihua Univ 2, 76–79.Google Scholar
Rajkhowa, R, Kaur, J, Wang, X and Batchelor, W (2015) Intrinsic tensile properties of cocoon silk fibres can be estimated by removing flaws through repeated tensile tests. J R Soc Interf 12(107), 20150177.CrossRefGoogle ScholarPubMed
Seydel, T, Kölln, K., Krasnov, I, Diddens, I, Hauptmann, N, Helms, G, Ogurreck, M, Kang, S-G, Koza, MM and Müller, M (2007) Silkworm silk under tensile strain investigated by synchrotron X-ray diffraction and neutron spectroscopy. Macromolecules 40(4), 1035–1042.CrossRefGoogle Scholar
Shao, Z (2015) Mulberry Silk, Spider Silk and Silk Protein. Beijing, China: Chemical Industry Press. (in Chinese).Google Scholar
Shao, Z and Vollrath, F (2002) Materials: Surprising strength of silkworm silk. Nature 418(6899), 741–741.CrossRefGoogle Scholar
Vollrath, F, Madsen, B and Shao, Z (2001) The effect of spinning conditions on the mechanics of a spider’s dragline silk. Proc R Soc B Biol Sci 268(1483), 2339–2346.CrossRefGoogle ScholarPubMed
Voronina, N (1994) Acoustic properties of fibrous materials. Appl Acou 42(2), 165–174.CrossRefGoogle Scholar
Wang, L (2009) A comparative study of the spider silk and mulberry silk. Jiangsu Silk 38(3), 5–6 (in Chinese).Google Scholar
Xia, Q, Li, S and Feng, Q (2014) Advances in silkworm studies accelerated by the genome sequencing of Bombyx mori . Annu Rev Entomol 59, 513–536.CrossRefGoogle ScholarPubMed
Yang, Y, Chen, X, Zhou, P, Yao, W-H and Shao, Z-Z (2001) Mechanical properties of Bombyx mori silk at different temperatures. Chem J Chin Univ 22(9), 1592–1596.Google Scholar
Zhang, K, Si, F-W, Duan, H-L and Wang, J (2010) Microstructures and mechanical properties of silks of silkworm and honeybee. Acta Biomater 6(6), 2165–2171.CrossRefGoogle ScholarPubMed
Zhang, Y-H, Xu, A-Y, Li, M-W, Sun, P-J and Qina, H-Y (2006) The diversity of strength and elongation of cocoon filament and correlative analysis among silkworm races. Silk 43(7), 28–30.Google Scholar
Zhang, Y, Li, J, Zhao, F, Zhou, Y, Zheng, H and Wu, Z (2015) A comparison on structure of cocoon filaments from mulberry silkworm, eri-silkworm and giant silkworm. Sci Sericult 41(3), 491–497.Google Scholar
Zhang, Y, Zhao, A-C, Chen, X-J, Liu, C and Xiang, Z-H (2011) A new method of measuring the cross-sectional area of single silk fiber from silkworm cocoon. Sci Sericult 37(5), 942–947.Google Scholar
Zhao, H-P, Feng, X-Q and Shi, H-J (2007) Variability in mechanical properties of Bombyx mori silk. Mater Sci Eng C 27(4), 675–683.CrossRefGoogle Scholar
Zhou, Z-Y and Yang, D-H (2005) Measurement about silk sectional area based on computer vision technology. J Text Res 26(1), 36–38.Google Scholar