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A Simple and Efficient Method to Observe Internal Structures of Helminths by Scanning Electron Microscopy
Published online by Cambridge University Press: 22 October 2013
Abstract
Morphological studies of helminths by scanning electron microscopy are generally limited to the external topography of the organisms. In this work, we present a simple technique using ethanol as a cryoprotectant without postfixation in osmium tetroxide that allows for observation of the inner organization of helminths and preserves cellular structures. We tested the technique in three helminths: Echinostoma paraensei, Cruzia tentaculata, and Hassalstrongylus epsilon. The results show that this technique could potentially be applied to study the morphology, ultrastructure, and taxonomy of helminths.
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- Biomedical and Biological Applications
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- Copyright © Microscopy Society of America 2013
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