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Scripted Image Enhancement / Analysis Using Adobe® Photoshop® With Ip Tools® Plug-Ins

Published online by Cambridge University Press:  02 July 2020

R. Underwood ,
L. Muffley ,
H. Predd ,
M. Piepkorn  and
J. Olerud
R. Underwood
Affiliation:
Department of Medicine (Dermatology), University of Washington, Seattle, WA
L. Muffley
Affiliation:
Department of Medicine (Dermatology), University of Washington, Seattle, WA
H. Predd
Affiliation:
Department of Medicine (Dermatology), University of Washington, Seattle, WA
M. Piepkorn
Affiliation:
Department of Medicine (Dermatology), University of Washington, Seattle, WA
J. Olerud
Affiliation:
Department of Medicine (Dermatology), University of Washington, Seattle, WA
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Extract

The Derm Imaging Center uses various light and electron microscopic techniques to determine meaningful morphologic differences in skin samples received from multiple investigators. Qualitative comparisons required laborious hand measurements, which, in time gave way to computer assisted image capture, enhancement and analysis. The ability to attach a numerical value to a morphologic observation has made quantification an expected component of the experimental results. Laboratory personnel are now spending more time in front of computers than microscopes. This fact prompted a reevaluation of our current methods resulting in a faster, user-friendly computer assisted imaging system.

One software program that has become ubiquitous within the field of scientific imaging is Adobe® Photoshop®. Although a multitude of different image capture and analysis software programs are in use, a version of Photoshop® can invariably be found residing in most laboratories involved in imaging. A disadvantage of many image analysis software programs is the lack of image enhancement capability that we have grown to know and love within the Photoshop® environment.

Type
Highlights of Biological Microscopy In The Pacific Northwest Usa
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1238 - 1239
Copyright
Copyright © Microscopy Society of America

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References

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2. Smith, L. T. et al., Mutations in the COL3A1 Gene Result in Alterations in the Size and Distribution of the Major Collagen Fibrils of the Dermis, J. Investigative Dermatology. 108(1997)241.CrossRefGoogle ScholarPubMed