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SCFV-6HIS Bioengineered for High Fidelity Labeling.

Published online by Cambridge University Press:  02 July 2020

Marek Malecki ,
Lea Tiongco ,
Annie Hsu  and
Nina Takeuchi
Marek Malecki
Affiliation:
Molecular Imaging Laboratories, University of California, San Diego, California.
Lea Tiongco
Affiliation:
Molecular Imaging Laboratories, University of California, San Diego, California.
Annie Hsu
Affiliation:
Molecular Imaging Laboratories, University of California, San Diego, California.
Nina Takeuchi
Affiliation:
Molecular Imaging Laboratories, University of California, San Diego, California.
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Extract

In life sciences, the essential part of the functional molecular analysis is the unambiguous identification of biochemical composition of the observed structures. This analysis is expected to create a bridge between functional data pouring from biochemistry and molecular biology laboratories with the molecular architecture data available from ultrastructural images. This goal can be attained by application of various ultrastructural tags (See: Albrecht et al. 1992).

A new promising approach for high fidelity labeling is offered by molecular cloning and expression of molecules containing metal binding sites making them suitable for electron spectroscopic imaging (ESI) (Malecki 1995). A truly enormous potential of ESI relies in its ability for mapping of various elements within the same sample. Interactions of electrons with an atom result in the electrons specific energy loss. Based upon these energy losses distribution of the elements within the sample can be mapped.

Type
Labeling for Microscopy and Correlative Microscopy
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 338 - 339
Copyright
Copyright © Microscopy Society of America

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References

Albrecht, et al. 1992. Methods Enzymol. 215:456-79.CrossRefGoogle Scholar
Kessels, et al. 1995. Scanning Microscopy Suppl. 10:327-44.Google Scholar
Malecki, 1995. Scanning Microscopy Suppl. 10:1–26.Google Scholar
Malecki, et al. 1998. Microscopy and Microanalysis: 994-5. San Francisco Press Inc., San Francisco, CA.Google Scholar