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Scanning Electron Microscopy of the Endometrium of Mares Infused with Gentamicin

Published online by Cambridge University Press:  17 March 2004

F.K. Al-Bagdadi ,
B.E. Eilts  and
G.F. Richardson
F.K. Al-Bagdadi
Affiliation:
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
B.E. Eilts
Affiliation:
Department of Veterinary Clinical Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
G.F. Richardson
Affiliation:
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, P.E.I. C1A 4P3, Canada
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Abstract

Scanning electron microscopy (SEM) was used to study the endometrium of nine 1-year-old thoroughbred mares after twice intrauterine infusions of gentamicin, on 2 consecutive days. Five mares were infused on 2 consecutive days with 40 ml gentamicin (50 mg/ml) mixed with 80 ml of normal saline. Four mares served as controls and were infused with 120 ml of saline on 2 consecutive days. Endometrial biopsies were obtained from all mares 3 days after the second intrauterine infusion. Each biopsy was processed for SEM by standard methods. The endometrial epithelium of the gentamicin-infused mares had more cellular perforations than the saline-infused mares. The gentamicin-infused mares had less and shorter microvilli. The ciliated cells were fewer and some ciliated cells had disrupted and some had drooping cilia. The endometrial epithelium of the gentamicin-infused mares had a considerable number of endometrial cells that lost their luminal surfaces and some that lost their microvilli, compared to the saline-infused mares. We suggest that the information gathered in this pilot study should be used as basis for further investigation, on a larger scale basis, of the effects of repeated intrauterine infusion of gentamicin on the endometrial mucosa of mares.

Keywords

endometriumgentamicinmaresuterus
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 10 , Issue 2 , April 2004 , pp. 280 - 285
Copyright
© 2004 Microscopy Society of America

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References

REFERENCES

Blanchard, T.L. & Woods, G.L. (1980). Reproductive management of the barren mare. Comp Cont Educ Pract Vet 2, S141–S148.Google Scholar
D'Aquino, S. & Pulle, C. (1970). Scanning electron microscopic studies on some ultrastructural characteristics of the human endometrium and ovary. Archive di Ostetricia e Gynecology 75, 267–282.Google Scholar
Eilts, B.E., McCoy, D.J., Taylor, H.W., Al-Bagdadi, F.K., Hansen, P.N., & Moser, E.B. (1988). Effect of repeated intrauterine infusions of gentamicin on the equine endometrium. Theriogenology 29, 1253–1259.Google Scholar
Gustafson, B.K. & Ott, R.S. (1981). Current trends in the treatment of genital infections in large animals. Comp Cont Educ Pract Vet 3, 105–109.Google Scholar
Houdeshell, J.W. & Hennessey, P.W. (1972). Gentamicin in the treatment of equine Metritis. VM/SAC 67, 1348–1352.Google Scholar
Johannisson, E. & Nelson, L. (1972). Scanning electron microscopic study of the human endometrium. Fertil Steril 23, 613–625.Google Scholar
Kanagawa, H., Hafez, E.S.E., Pitchford, W.C., Baechler, C.A., & Barnhart, M.I. (1972). Surface patterns in the reproductive tracts of the rabbit observed by scanning electron microscopy. Anat Rec 174, 205–226.Google Scholar
Kenny, R.M. (1978). Cyclic and pathological changes of the mare endometrium as detected by biopsy with a note on early embryonic death. J Am Vet Med Assoc 172, 241–262.Google Scholar
Kojima, Y.A. & Slander, E.N. (1970). Cyclic changes in the fine structure of bovine endometrial gland cells. Zellforsch Mikrosk Z 104, 69–86.Google Scholar
Lieux, P. (1972). Reproduction and genital diseases. Equine Medicine and Surgery, 2nd ed., Catcott, E.J. & Smithcors, J.F. (Eds.), pp. 597–654. Wheaton: American Veterinary Publications, Inc.
More, I.A.R. & Masterson, R.G. (1976). The role of oestrogen in the control of ciliated cells of the human endometrium. J Reprod Fert 47, 19–24.Google Scholar
Nelson, O. (1962). Electron microscopy of the glandular epithelium in human uterus. II. Early and late luteal phase. J Ultarast Res 6, 422–431.Google Scholar
Olsen, L.M., Al-Bagdadi, F.K., Richardson, G.F., Archbald, L.F., Braun, W.F., McCoy, D.J., Godke, R.A., Titkemeyer, C.W., & Thompson, D.L. (1992). A histological study of the effect of saline and povidone-iodine infusions on the equine endometrium. Theriogenology 37, 1311–1325.Google Scholar
Ricketts, S.W., Rossdale, P.D., & Samuel, C.A. (1978). Endometrial biopsy studies of mares with contagious equine Metritis. Equine Vet J 10, 160–166.Google Scholar
Samuel, C.A., Ricketts, S.W., Rossdale, P.D., Steven, D.H., & Thurley, K.W. (1979). Scanning electron microscope studies of the endometrium of the cyclic mare. J Reprod Fert Suppl 27, 287–292.Google Scholar
Sanfilippo, J.S., Barrows, G.H., Apharian, R.P., & Wittliff, J.L. (1985). Evaluation of damazol influence upon the uterus using scanning electron microscope morphometric and biochemical analysis. Surg Gynecol Obstet 16, 421–428.Google Scholar
Wyn, R.M., Harris, J.A., & Wooley, R.S. (1967). Ultrastructural cyclic changes in the human endometrium II Normal postovulatory phase. Fertil Steril 18, 721–726.Google Scholar