Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-29T23:23:51.935Z Has data issue: false hasContentIssue false
  • English
  • Français

The Response of Maize Protoplasts to High Intensity Illumination in Multi-Photon Fluorescence Microscopy

Published online by Cambridge University Press:  02 July 2020

B. L. Lin ,
F. J. Kao ,
P. C. Cheng  and
P. C. Cheng
B. L. Lin
Affiliation:
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, 11529 Rep. of China
F. J. Kao
Affiliation:
department of Physics, National Sun Yat-sen University, Kaohsiung, Taiwan, 80424 Rep. of China
P. C. Cheng
Affiliation:
AMIL, Dept. of Electrical Engineering, State University of new York, Buffalo, NY14260USA
P. C. Cheng
Affiliation:
Williamsville East HS, Williamsville, NY14051USA
Get access

Extract

Multi-photon fluorescence microscopy has been cited for its advantage in increased depth penetration due to low linear absorption coefficient of biological specimen in the near infrared (NIR) range. Using a pulsed laser, it is possible to efficiently excite two-photon fluorescence with a high peak power while keeping the average power low to avoid thermal and photochemical damages to the specimen. Currently, mode-locked Ti-sapphire and Cr-Forsterite lasers that generate sub-picosecond pulses are used as the light source for multi-photon fluorescence microscopy. Because of the need of high peak power for efficiently exciting two-photon fluorescence, the relationship between cell damage and peak power has become an interesting and much debated topic in the application of multi-photon fluorescence microscopy. It is conceivable that at high illumination intensity, non-linear photochemical processes may have impacts on cell physiology and viability in ways much different from low illumination in the linear domain.

Type
Advances in Multi-Photon Imaging
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 806 - 807
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Cheng, P. C. et al., Micron, 2000, in pressGoogle Scholar

2. Konig, K. et al., Optics Lett. 22: 135–136, (1997).CrossRefGoogle Scholar

3. Huang, H. C. and Chen, C. -C, Heredity, J. 79, 28–32, (1988).CrossRefGoogle Scholar

4. Kao, F. J. et al., SPIE Proceedings, 3919, 2000, in press.Google Scholar

5. Cheng, P. C. et al, Maize Genetic Cooperation Newsletter, 74, (2000)Google Scholar

Supported by Academia Sinica (BLL), Natl. Sci. Council, Rep. of China [NSC-89-2311-B-001-032 (BLL), NSC-88-2311-B-OO 1-087 (FJK), NSC-88-2311-B-OO 1-087 (PCC)] and Mr. and Mrs. Jin-Mu Huang of Aurum Belle Investment Co., (on behalf of the Ge-An Charity, ).Google Scholar