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Preservation of Articular Cartilage for Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

Iolo ap Gwynn ,
Geoffery Richards  and
Stephen Wade
Iolo ap Gwynn
Affiliation:
Institute of Biological Sciences, The University of Wales, Aberystwyth, Ceredigion, , SY23 3DA, Wales
Geoffery Richards
Affiliation:
AO Research Institute, Clavadelerstrasse, CH7270, Davos Platz, Switzerland
Stephen Wade
Affiliation:
Institute of Biological Sciences, The University of Wales, Aberystwyth, Ceredigion, , SY23 3DA, Wales
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Abstract

Water makes up 60-80% of the wet weight of articular cartilage. The remaining parts of this tissue are made up of a variety of cells, generally referred to as chondrocytes, and an extracellular matrix of, collagen fibrils (types II, IX and XI), proteoglycans. The collagens and proteoglycans, with associated water, are assembled into a structure that is capable of withstanding considerable loading.

The micro-structure of adult rabbit articular cartilage has been described, based upon ultrastructural studies. The tissue is arranged into zones, mainly based on the general orientation of collagen fibrils (See Figure 1). The collagen framework in the radial zone forms a continuous area of radially oriented and tightly bound collagen fibrils, in which are embedded at regular interval, tubular inclusions of proteoglycans (Figure 2). in rabbit tibial plateau tissue these inclusions have diameter of 1-3 fim and can be continuous from the calcified to the tangential zone.

Type
Cryoimmobilization, Freeze Substitution and Cryoem (Organized by S. Erlandsen)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 726 - 727
Copyright
Copyright © Microscopy Society of America 2001

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References

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5)This work was supported by grant No 98/G36 from the AO/ASIF-Foundation, Switzerland.Google Scholar