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The Precise Alignment and Auto-fusion of Correlative Cryo-SXT and Cryo-FM.

Published online by Cambridge University Press:  10 August 2018

Haobo Bai
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Yong Guan
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Liang Chen
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Gang Liu
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
Yangchao Tian*
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China
*
*Yangchao Tian, [email protected]

Abstract:

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Cell's sub-cellular architecture and function can be revealed by Cryo soft X-ray tomography (Cryo-SXT) and Cryo-fluorescence microscopy (Cryo-FM) respectively. To understand the connection between cells' structure and function, correlative Cryo-SXT and Cryo-FM has been applied. Here we introduce a semiautomatic precise alignment and fusion technology of Cryo-SXT and Cryo-fluorescence microscopy.

Type
Abstract
Copyright
© Microscopy Society of America 2018 

References

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