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Postnatal Developmental Changes in Fractal Complexity of Giemsa-Stained Chromatin in Mice Spleen Follicular Cells

Published online by Cambridge University Press:  18 September 2017

Igor Pantic Open the ORCID record for Igor Pantic [Opens in a new window] ,
Jovana Paunovic ,
Danijela Vucevic ,
Tatjana Radosavljevic ,
Stefan Dugalic ,
Anita Petkovic ,
Sanja Radojevic-Skodric  and
Senka Pantic
Igor Pantic*
Affiliation:
Laboratory for Cellular Physiology, Institute of Medical Physiology, Faculty of Medicine, University of Belgrade, Visegradska 26/II, RS-11129 Belgrade, Serbia University of Haifa, 199 Abba Hushi Blvd, Mount Carmel, Haifa 3498838, Israel
Jovana Paunovic
Affiliation:
Institute of pathological physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 9, RS-11129 Belgrade, Serbia
Danijela Vucevic
Affiliation:
Institute of pathological physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 9, RS-11129 Belgrade, Serbia
Tatjana Radosavljevic
Affiliation:
Institute of pathological physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 9, RS-11129 Belgrade, Serbia
Stefan Dugalic
Affiliation:
Clinic for Gynecology and Obstetrics, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Dr Koste Todorovica 26, RS-11000 Belgrade, Serbia
Anita Petkovic
Affiliation:
Clinic for infectious diseases, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Bulevar oslobodjenja 16, RS-11000 Belgrade, Serbia
Sanja Radojevic-Skodric
Affiliation:
Institute of Pathology, Faculty of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia
Senka Pantic
Affiliation:
Institute of Histology and Embryology, Faculty of Medicine, University of Belgrade, Visegradska 26/II, RS-11129 Belgrade, Serbia
*
*Corresponding author. [email protected]
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Abstract

Although there are numerous recent works focusing on fractal properties of DNA and chromatin, many issues regarding changes in chromatin fractality during physiological aging remain unclear. In this study, we present results indicating that in mice, there is an age-related reduction of chromatin fractal complexity in a population of spleen follicular cells (SFCs). Spleen tissue was obtained from 16 mice and fixated in Carnoy solution. The youngest animal was newborn, and each animal was exactly 1 month older than the previous. We performed fractal analysis of SFC chromatin structure, stained using Giemsa technique. Fractal analysis was done in a plugin algorithm of ImageJ software. We also performed gray-level co-occurrence matrix (GLCM) analysis of all chromatin structures with the calculation of parameters such as angular second moment and inverse difference moment. Giemsa-stained SFC chromatin exhibited an age-dependent reduction of fractal dimension with statistically significant (p<0.01) linear trend. Moreover, there was a statistically significant increase of SFC chromatin lacunarity. The chromatin GLCM parameters did not significantly change. To our knowledge, this is the first study to perform fractal and GLCM analyses of SFC chromatin and to investigate potential changes of fractal parameters during postnatal development.

Keywords

spleenagingmicroscopymicrographlymphocyte
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 5 , October 2017 , pp. 1024 - 1029
Copyright
© Microscopy Society of America 2017 

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