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Placing TEM Negative Images into the Digital Domain
Published online by Cambridge University Press: 02 July 2020
Abstract
Several thousand images of various biological tissues have been produced by these investigators (*) over careers spanning many, many years. The majority of the images are preserved on Kodak Electron Image Film #4463, Kodak Electron Microscopy Film #4489, Kodak Electron Image Film #SO 163, or Dupont Ortho S Litho (COS-7) film. These 7 mil ESTAR thick base films were the standards utilized by the biological microscopist. The emulsion was covered with a thin layer of hardened gelatin in order to minimize abrasion. After exposure to the electron beam in the 60-80 kV range, the negatives were developed according to routine photographic procedures employing Kodak D-19 developer, hardened in Kodak fixer, washed for the appropriate time, and stored in glassine envelopes in a dust-free environment. The finished negative with the captured image proves to be long-lasting and will produce an image, today, displaying the very same excellent qualities as did original prints produced years ago.
- Type
- Ask the Experts: Addressing Issues in Digital Imaging for the Microscopist (Organized by J. Mascorro, R. Anderson and D. Sherman)
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- Copyright © Microscopy Society of America 2001