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Phase Contrast Synchrotron Microtomography: Improving Noninvasive Investigations of Fossil Embryos In Ovo

Published online by Cambridge University Press:  18 January 2012

Vincent Fernandez ,
Eric Buffetaut ,
Eric Maire ,
Jérôme Adrien ,
Varavudh Suteethorn  and
Paul Tafforeau
Vincent Fernandez*
Affiliation:
European Synchrotron Radiation Facility, X-Ray Imaging Group, 6 rue Horowitz BP 220, 38046 Grenoble Cedex, France
Eric Buffetaut
Affiliation:
CNRS (UMR 8538), Laboratoire de Géologie de l'École Normale Supérieure, 24 rue Lhomond, 75231 Paris Cedex 05, France
Eric Maire
Affiliation:
Université de Lyon, INSA-Lyon, MATEIS CNRS (UMR 5510), 25 avenue jean Capelle, 69621, Villeurbanne, France
Jérôme Adrien
Affiliation:
Université de Lyon, INSA-Lyon, MATEIS CNRS (UMR 5510), 25 avenue jean Capelle, 69621, Villeurbanne, France
Varavudh Suteethorn
Affiliation:
Bureau of Fossil Research and Museum, Department of Mineral Resources, Rama VI Road, Bangkok 10400, Thailand
Paul Tafforeau
Affiliation:
European Synchrotron Radiation Facility, X-Ray Imaging Group, 6 rue Horowitz BP 220, 38046 Grenoble Cedex, France
*
Corresponding author. E-mail: [email protected]
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Abstract

Fossil embryos are paramount for our understanding of the development of extinct species. However, although thousands of fossil amniote eggs are known, very few embryos in ovo have been described. First reports of fossil embryos were based on broken eggs, where the embryonic remains were already exposed, because destructive methods on complete eggs were avoided. Investigations of complete eggs therefore required nondestructive approaches, such as X-ray microtomography (μCT). However, due to the general low density contrast between fossilized bones and infilling matrix, only a few specimens have been reported using these techniques. Using propagation phase contrast X-ray synchrotron microtomography (PPC-SR-μCT), we report here the discovery of three well-preserved embryos in Early Cretaceous eggs from Thailand. By scanning these eggs using different imaging techniques, we show that vastly different interpretations can be made regarding the preservation state and/or the developmental stage of these embryos. PPC-SR-μCT also revealed differential contrast between bone categories, presumably reflecting the ossification pattern of these embryos. Applying such an approach to large-scale studies of fossil eggs could lead to more discoveries and detailed studies of fossil embryos, providing important developmental and phylogenetic information on extinct and extant amniotes.

Keywords

phase contrastmicrotomographyfossil eggfossil embryo
Type
Techniques Development
Information
Microscopy and Microanalysis , Volume 18 , Issue 1 , February 2012 , pp. 179 - 185
Copyright
Copyright © Microscopy Society of America 2012

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Footnotes

Current address: Bernard Price Institute for Palaeontological Research, University of the Witwatersrand, Private Bag 3, Johannesburg 2050, South Africa

References

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