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Ovarian Carcinomas: Insights into Origins Using Confocal Microscopy and Fluorescence in Situ Hybridization on Intact Paraffin Sections

Published online by Cambridge University Press:  02 July 2020

N. G. Wolf ,
F. W. Abdul-Karim ,
N. J. Schork  and
S. Schwartz
N. G. Wolf
Affiliation:
Department of Genetics and Center for Human Genetics, Case Western Reserve University and University Hospitals of Cleveland, 10900 Euclid Avenue, Cleveland, OH, 44106
F. W. Abdul-Karim
Affiliation:
Institute of Pathology, Case Western Reserve University, 2088 Adelbert, Cleveland, OH, 44106
N. J. Schork
Affiliation:
Department of Epidemiology and Biostatistics, Case Western Reserve University, Cleveland, OH, 44106
S. Schwartz
Affiliation:
Department of Genetics and Center for Human Genetics, Case Western Reserve University and University Hospitals of Cleveland, 10900 Euclid Avenue, Cleveland, OH, 44106
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Extract

Although ovarian carcinomas are the most lethal gynecologic tumors, their origins remain unclear. Do they develop from malignant transformation of benign neoplasms through a multistep process of tumor progression, or do they arise de novo? The histologically benign and/or low malignant potential (LMP) components in heterogeneous ovarian carcinomas have been considered as evidence supporting the theory of tumor progression. These components are interpretted as the remnants of pre-existing neoplasms that underwent malignant transformation. In two other possible interpretations, however, such components may be clones which developed independently (de novo hypothesis) or they may represent malignant epithelium which underwent focal maturation (maturation hypothesis).

To evaluate genetic relationships of the histological components in heterogeneous ovarian carcinomas, 10 such neoplasms and 5 normal ovary controls were examined using fluorescence in situhybridization (FISH) on intact paraffin sections (6 μm).The retention of tissue architecture allowed direct correlation of detectable genetic aberrations with histology, and comparison of malignant components with adjacent histologically benign or LMP components.

Type
Neoplasia: Abnormal Cell Growth or Death/Apoptosis? Insights from Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 5 - 6
Copyright
Copyright © Microscopy Society of America 1997

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References

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8. This research was supported by a National Research Service Award (CA-67515-01) from the National Institutes of Health, a Cancer Resarch Fellowship from the American Cancer Society (Ohio Division), and the Norma Geller Ovarian Cancer Research Fund of the Ireland Cancer Center of University Hosptials of Cleveland.Google Scholar