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Optimizing F-actin Labeling At the Leading Edge Of Cells Using Multiple Actin Probes, Fixation Methods and Imaging Techniques

Published online by Cambridge University Press:  05 August 2019

Vera DesMarais ,
Robert J. Eddy ,
Ved P. Sharma ,
Orrin Stone  and
John S. Condeelis
Vera DesMarais
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA. Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY, USA. Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.
Robert J. Eddy
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA.
Ved P. Sharma
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA.
Orrin Stone
Affiliation:
Department of Pharmacology, UNC Chapel Hill School of Medicine, Chapel Hill, NC, USA.
John S. Condeelis
Affiliation:
Department of Anatomy and Structural Biology/Albert Einstein College of Medicine, Bronx, NY, USA. Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY, USA. Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.

Abstract

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Type
Light and Fluorescence Microscopy for Imaging Cell Surface and Cell Structure
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1254 - 1255
Copyright
Copyright © Microscopy Society of America 2019 

References

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[8]All imaging was conducted in the Analytical Imaging Facility (AIF) (funded by NCI Cancer Grant P30CA013330). SIM imaging was executed on the Nikon-STORM/SIM/TIRF microscope (funded by NIH 1S10OD18218-1). Some confocal imaging was executed on the Leica SP8 confocal microscope (funded by NIH 1S10OD023591-01). The research was funded by grant CA150344 (RE, VS, JC).Google Scholar