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Optimization of Microfluidics for Genetic Analysis

Published online by Cambridge University Press:  02 July 2020

Pamela M. St. John ,
Kip Connell ,
T. M. Woudenberg ,
M. Deshpande  and
J. R. Gilbert
Pamela M. St. John
Affiliation:
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA, 94404
Kip Connell
Affiliation:
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA, 94404
T. M. Woudenberg
Affiliation:
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA, 94404
M. Deshpande
Affiliation:
2Microcosm Technologies, Inc., 215 1, stSt., Cambridge, MA, 02142
J. R. Gilbert
Affiliation:
2Microcosm Technologies, Inc., 215 1, stSt., Cambridge, MA, 02142
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Extract

Novel microfluidic designs are needed for fluid delivery and transport in an integrated micro-genetic analysis system and these designs often lead to complex relationships between the shape and size of the microchannels and the behavior of multi-component fluids. We are studying both pressure-driven and electrokinetic flow in various microfluidic channel designs fabricated from both glass and silicone substrates to understand chemical containment and transport. In conjunction with the experiments, simulations are being performed to predict behavior in microdevices and models are being used to develop a CAD tool for designers.

Fluorescent flow profiles are generated using a charged caged dye which fluoresces upon irradiation with 355 nm light from a Nd:YAG laser. The starting position of the dye is determined by a tightly focused laser spot <20 μm in diameter.

Type
Miniaturized Artificial Machines in Biology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 892 - 893
Copyright
Copyright © Microscopy Society of America

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