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New Viruses from Lacerta monticola (Serra da Estrela, Portugal): Further Evidence for a New Group of Nucleo-Cytoplasmic Large Deoxyriboviruses

Published online by Cambridge University Press:  08 December 2010

António Pedro Alves de Matos ,
Maria Filomena Alcobia da Silva Trabucho Caeiro ,
Tibor Papp ,
Bruno André da Cunha Almeida Matos ,
Ana Cristina Lacerda Correia  and
Rachel E. Marschang
António Pedro Alves de Matos*
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal CESAM—Centre for Environmental and Marine Studies. Aveiro University, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
Maria Filomena Alcobia da Silva Trabucho Caeiro
Affiliation:
CESAM—Centre for Environmental and Marine Studies. Aveiro University, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal University of Lisbon, Faculty of Sciences, Department of Plant Biology, Campo Grande, 1749-016 Lisbon, Portugal
Tibor Papp
Affiliation:
Institut für Umwelt und Tierhygiene, Hohenheim University, Garbenstr. 30, 70599 Stuttgart, Germany
Bruno André da Cunha Almeida Matos
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal
Ana Cristina Lacerda Correia
Affiliation:
Anatomic Pathology Department, Curry Cabral Hospital, R. da Beneficência 8, 1069-166 Lisboa, Portugal
Rachel E. Marschang
Affiliation:
Institut für Umwelt und Tierhygiene, Hohenheim University, Garbenstr. 30, 70599 Stuttgart, Germany
*
Corresponding author. E-mail: [email protected]
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Abstract

Lizard erythrocytic viruses (LEVs) have previously been described in Lacerta monticola from Serra da Estrela, Portugal. Like other known erythrocytic viruses of heterothermic vertebrates, these viruses have never been adapted to cell cultures and remain uncharacterized at the molecular level. In this study, we made attempts to adapt the virus to cell cultures that resulted instead in the isolation of a previously undetected Ranavirus closely related to FV3. The Ranavirus was subsequently detected by polymerase chain reaction (PCR) in the blood of infected lizards using primers for a conserved portion of the Ranavirus major capsid protein gene. Electron microscopic study of the new Ranavirus disclosed, among other features, the presence of intranuclear viruses that may be related to an unrecognized intranuclear morphogenetic process. Attempts to detect by PCR a portion of the DNA polymerase gene of the LEV in infected lizard blood were successful. The recovered sequence had 65.2/69.4% nt/aa% homology with a previously detected sequence from a snake erythrocytic virus from Florida, which is ultrastructurally different from the studied LEV. These results further support the hypothesis that erythrocytic viruses are related to one another and may represent a new group of nucleo-cytoplasmic large deoxyriboviruses.

Keywords

lizard erythrocytic virusRanavirusLacerta monticolavirus isolationPCR
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 1 , February 2011 , pp. 101 - 108
Copyright
Copyright © Microscopy Society of America 2011

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