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New Single Molecule Approaches to Genomic Analysis: Optical Mapping

Published online by Cambridge University Press:  02 July 2020

Schwartz. D.C ,
Anantharaman T ,
Cai W ,
Clarke V ,
Delobette S ,
Dimalanta E ,
Edington J ,
Giacalone J ,
Hiort C  and
Hu X
...Show all authors
Schwartz. D.C
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Anantharaman T
Affiliation:
Courant Institute of Mathematical Sciences, Department of Computer Science, New York University, 251 Mercer Street, New York, NY10003
Cai W
Affiliation:
presently located at: Baylor College of Medicine, One Baylor Plaza, Houston, TX77030
Clarke V
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Delobette S
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Dimalanta E
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Edington J
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Giacalone J
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Hiort C
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Hu X
Affiliation:
presently located at:, CuraGen Corporation, 322 E. Main Street, Branford, CT06405
Huff E
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Jing J
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Lai Z
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Lee E
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Mishra B
Affiliation:
Courant Institute of Mathematical Sciences, Department of Computer Science, New York University, 251 Mercer Street, New York, NY10003
Murti J.R
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Porter B.
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Qi R
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Rabbah R
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Ramanathan A
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Reed J
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Samad A
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Shenker A
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Skiadas Y
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Tankhoveya K
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Wang W
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
Lin J
Affiliation:
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY10003
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Extract

Current molecular biological approaches were developed primarily for characterization of single genes, not entire genomes, and, as such, are not ideally suited to analysis of complex traits and population-based molecular genetics. Despite rapid progress in the human genome project effort, there is little doubt that radically new conceptual approaches are needed before routine whole genome-based analyses can be undertaken by both basic research and clinical laboratories.

Physical mapping of genomes, using restriction endonucleases, has played a major role in the identification and characterizing various loci, for example, by aiding clone contig formation and by characterizing genetic lesions. Restriction maps provide precise genomic distances, unlike ordered sequence-based landmarks such as Sequence Tagged Sites (STSs), that are essential for optimizing the efficiency of sequencing efforts, and for determining the spatial relationships of specific loci. When compared to tedious hybridization-based fingerprinting approaches, ordered restriction maps offer relatively unambiguous clone characterization that is useful in contig formation, establishment of minimal tiling paths for sequencing, and preliminary characterization of sequence lesions.

Type
Genomes, Chromsomes and Genes: Emerging Technologies
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 199 - 200
Copyright
Copyright © Microscopy Society of America 1997

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