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The New EM: Specimen Preparation Procedures for Resin Embedding of Cryofixed Biological Samples in 6 Hours (Without a Microwave)

Published online by Cambridge University Press:  27 August 2014

Kent L. McDonald*
Affiliation:
Electron Microscopy Laboratory, University of California, Berkeley, USA

Abstract

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Type
Abstract
Copyright
Copyright © Microscopy Society of America 2014 

References

[1] McDonald, K., Webb, R. J. Microscopy 243 (2011) p. 227.Google Scholar
[2] McDonald, K., Microanal, Microsc. doi:10.1017/S1431927613013846, Published online Nov. 19, 2013.Google Scholar
[3] McDonald, K. Protoplasma doi: 10.1007/s00709-013-0575-y, Published online Nov. 21, 2013.Google Scholar
4) The author wishes to thank the following individuals for providing materials for this work: Michael Melzer, Leibnitz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany for the Arabidopsis pollen, and Denise Lapidus and Barbara Meyer, University of California, Berkeley for the C. elegans worms. Thanks also to Richard Webb, Microscopy and Microanalysis Centre, University of Queensland, Australia for his continuing aid in the development of these rapid specimen preparation methods.Google Scholar