Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T15:45:23.796Z Has data issue: false hasContentIssue false
  • English
  • Français

Mouse Sperm Association During Epididymal Transit

Published online by Cambridge University Press:  02 July 2020

M. Monclus ,
M. H. Burgos  and
M.W. Fornés
M. Monclus
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
M. H. Burgos
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
M.W. Fornés
Affiliation:
Institute of Histology and Embriology. School of Medicine. National University of Cuyo -CONICET. P.O.Box (Casilla de Correo) 56. Mendoza. ZC 5500. Argentina. [email protected].
Get access

Extract

Head-to-head sperm association was described in some species as a normal phenomenon during epididyma transit (Phillips and Bedford, 1987). Recently, a new sperm head association (SHA) was characterized in the rat (Fornés & Burgos 1990; 1994).

At this moment, the study of SHA was extended to mouse sperm. SHA is a type of cell-cell interaction clearly observed when an a drop of epididymal fluid, obtained by a puncture of epididymal duct, is placed in a culture medium (HMB media: Modified Ringers medium).

This preparation can be observed under contrast phase microscope (optiphot-2, Nikkon) with or without fixative (2% paraformaldehyde and 2% glutaraldehyde in 0.01 M PBS buffer, pH 7,2). The mass of spermatozoa begin to move but not as a single sperm. They move as a clump of sperm and without fixative the sperm motility alone disaggregate the SHA.

Type
Developmental/Reproductive Biology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 968 - 969
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

Phillips, D. M. and Bedford, J.M.. Gamete Res. 18 (1988): 17–25.CrossRefGoogle Scholar
Fornes, M. W. y Burgos, M. H.. Mia. Electr. Biol. Cel. 14 (2) (1990): 115–130.Google Scholar
Fornes, M.W. y Burgos, M.H.. Mol. Reprod. Develop.: 38 (1994): 43–47.CrossRefGoogle Scholar