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Morphometrical Ultrastructural Studies of the Rat's Hepatocytes Under Cooling

Published online by Cambridge University Press:  02 July 2020

A.S. Kaprelyants ,
A.A. Kaprelyants ,
A.N. Reylan  and
R.K. Migunova
A.S. Kaprelyants
Affiliation:
Department of Cryocitology and Quantitative Morphology, Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, 310015 23 Pereyaslavskaya Str., Ukraine.
A.A. Kaprelyants
Affiliation:
Department of Cryocitology and Quantitative Morphology, Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, 310015 23 Pereyaslavskaya Str., Ukraine.
A.N. Reylan
Affiliation:
Department of Cryocitology and Quantitative Morphology, Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, 310015 23 Pereyaslavskaya Str., Ukraine.
R.K. Migunova
Affiliation:
Department of Cryocitology and Quantitative Morphology, Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, 310015 23 Pereyaslavskaya Str., Ukraine.
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Extract

The aim of given investigation is to study the effect of cooling upon rat hepatocyte structure using transmission electron microscopic and computer morphometric methods. Ultrastructural and morphometrical characteristics of hepatocytes under liver cooling for various levels under in vivo and in vitro conditions were investigated. Vistar rats of 180-250 g were used in the experiment. Liver cooling (in vivo) was performed by means of original cryoapplicator with different probe temperature (1,2). Liver tissue for transmission electron microscopy was fixed in glutaraldehyde fixator on cocadylate buffer and OsO4. Dehydration was completed on acetone (3). Tissue embedding was done into the mixture of Epon/Araldite epoxy rasin. Ultrathin slices were contrasted by the method of Reinolds. Cell viewing and imaging were accomplished by electron microscope at accelerating power of 75kV.

Morphometrical and stereometrical analysis was performed using the “Morpho-Tools” original computer system (c) 1994-1996 A.S. Kaprelyants, A.A. Kaprelyants, A.N. Reylan .

Type
Imaging Cells and Organelles
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 245 - 246
Copyright
Copyright © Microscopy Society of America 1997

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References

1. Programmable Cooling Device. USSR Pat. No. 19188.Google Scholar
2. Programmable Cooling Device. USSR Pat. No. 19189.Google Scholar
3.Device for Preparation of Biological Systems. GERMANY Pat. No. 262561Google Scholar