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Morphological Fractal Analysis of Shape in Cancer Cells Treated with Combinations of Microtubule-Polymerizing and -Depolymerizing Agents

Published online by Cambridge University Press:  22 June 2010

Sonal O. Uppal ,
Dmitri V. Voronine ,
Elizabeth Wendt  and
Carol A. Heckman
Sonal O. Uppal
Affiliation:
Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106, USA
Dmitri V. Voronine*
Affiliation:
Institut für Physikalische Chemie, Universität Würzburg, Würzburg 97074, Germany
Elizabeth Wendt
Affiliation:
Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA
Carol A. Heckman
Affiliation:
Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA
*
Corresponding author. E-mail: [email protected]
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Abstract

The current prognostic parameters, including tumor volume, biochemistry, or immunohistochemistry, are not sufficient to reflect the properties of cancer cells that distinguish them from normal cells. Our focus is to evaluate the effects of a combination of microtubule-polymerizing Taxol® and -depolymerizing colchicine on IAR20 PC1 liver cells by measuring the surface fractal dimension as a descriptor of two-dimensional vascular geometrical complexity. The fractal dimension offers a rapid means of assessing cell shape. Furthermore, we show correlations of fractal dimensions of cell contours with the latent factors from our previously employed cell shape analysis.

Keywords

cell shapecancermicrotubulefractal analysis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 4 , August 2010 , pp. 472 - 477
Copyright
Copyright © Microscopy Society of America 2010

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