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A Morphological and Cytochemical Study of the Interaction between Paracoccidiodes brasiliensis and Neutrophils

Published online by Cambridge University Press:  17 March 2004

Maria Fernanda R.G. Dias ,
Absalom L. Filgueira  and
Wanderley de Souza
Maria Fernanda R.G. Dias
Affiliation:
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS-Bloco G, 21949-900, Rio de Janeiro, RJ- Brasil
Absalom L. Filgueira
Affiliation:
Departamento de Dermatologia, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ- Brasil
Wanderley de Souza
Affiliation:
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS-Bloco G, 21949-900, Rio de Janeiro, RJ- Brasil
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Abstract

Paracoccidioidomycosis is a systemic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. It is the most prevalent systemic mycosis of Latin America and 80% of the reported cases are from Brazil. Because of the great number of neutrophils found in the P. brasiliensis granuloma, studies have been done to evaluate the role of these cells during the development of the infection. Scanning and transmission electron microscopy of thin sections showed that the neutrophils ingest yeast cells through a typical phagocytic process with the formation of pseudopodes. The pseudopodes even disrupt the connection established between the mother and the bud cells. Neutrophils also associate to each other, forming a kind of extracellular vacuole where large yeast cells are encapsulated. Cytochemical studies showed that once P. brasiliensis attaches to the neutrophil surface, it triggers a respiratory burst with release of oxygen-derived products. Attachment also triggers neutrophils' degranulation, with release of endogenous peroxidase localized in cytoplasmic granules. Together, these processes lead to killing of both ingested and extracellular P. brasiliensis.

Keywords

Paracoccidioides brasiliensisoxidative burstphagocytosisendogenous peroxidaseNAD(P)H-oxidasealkaline phosphataseacid phosphatase
Type
Papers from the InterAmerican Congress on Electron Microscopy
Information
Microscopy and Microanalysis , Volume 10 , Issue 2 , April 2004 , pp. 215 - 223
Copyright
© 2004 Microscopy Society of America

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