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Microstructural Changes of the Compact Bone in the Mandible of Aging Rats Using Plastic Casting Method

Published online by Cambridge University Press:  02 July 2020

S. Okada ,
F. Iwaku ,
S. Yoshida  and
Y. Ohta
S. Okada
Affiliation:
Department of Anatomy, Asahi University School of Dentistry, Motosu-gun, Gifu, 501-0296, Japan
F. Iwaku
Affiliation:
Department of Anatomy, Asahi University School of Dentistry, Motosu-gun, Gifu, 501-0296, Japan
S. Yoshida
Affiliation:
Department of Anatomy, Hokkaido University School of Dentistry, Sapporo060-8586, Japan
Y. Ohta
Affiliation:
Department of Anatomy, Osaka Dental University, Hirakata, Osaka, 573-1121, Japan
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Extract

A method for producing plastic castings of the internal structure of hard tissue has been described by Marten et al. (1978). Although they examined dentine, this technique could also be used to examine other hard tissue such as bone (Weber, 1983). The aim of our study is to expose the true threedimensional relationships between the internal structures of the compact bone and these microstructural changes of the mandible in the aging rats.

Nine Wistar male rats were used in this study and divided into 3 groups as follows: 3 weeks-old rats as young stage, 12 weeks-old as adult stage and 80 weeks-old as old stage. All rats were perfused with 2.5% glutaraldehyde via the ascending aorta. In one rat for each group, the mandible was decalcified by 10% EDTA and embedded in paraffin. 10 μm sections were stained with Schmorl’s staining method.

Type
Applications and Advances In Vascular Corrosion Casting In Microvascular Research
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1200 - 1201
Copyright
Copyright © Microscopy Society of America

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References

1.Marten, D. M. et al., J. Microsc. 112 (1978) 345.CrossRefGoogle Scholar
2.Weber, D. F.. Archs oral Biol. 28 (1983) 885.CrossRefGoogle Scholar
3.Yoshida, S. et al., Hokkaido J. Dent. Sci. 14 (1993) 140.Google Scholar