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Microscopy and Microanalysis of Blood in a Snake Head Fish, Channa gachua Exposed to Environmental Pollution

Published online by Cambridge University Press:  25 February 2016

Eva M. Pala  and
Sudip Dey
Eva M. Pala
Affiliation:
Department of Zoology, Shillong College, Shillong-793003, Meghalaya, India
Sudip Dey*
Affiliation:
Electron Microscope Division, Sophisticated Analytical Instrument Facility, North Eastern Hill University, Shillong-793022, Meghalaya, India
*
*Corresponding author. [email protected]
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Abstract

Conventional and highly sophisticated analytical methods (Cyria et al., 1989; Massar et al., 2012a) were used to analyze micro-structural and micro-analytical aspects of the blood of snake head fish, Channa gachua, exposed to municipal wastes and city garbage. Red (RBC) and white blood cell (WBC) counts and hemhemoglobin content were found to be higher in pollution affected fish as compared with control. Scanning electron microscopy revealed the occurrence of abnormal erythrocytes such as crenated cells, echinocytes, lobopodial projections, membrane internalization, spherocytes, ruptured cells, contracted cells, depression, and uneven elongation of erythrocyte membranes in fish inhabiting the polluted sites. Energy-dispersive X-ray spectroscopy (EDS) revealed the presence of silicon and lead in the RBCs of pollution affected fish. Significance of the study includes the highly sophisticated analytical approach, which revealed the aforementioned micro-structural abnormalities.

Keywords

Channa gachuahill streamcity garbageerythrocytes
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 1 , February 2016 , pp. 39 - 47
Copyright
© Microscopy Society of America 2016 

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