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Micropatterning of electron microscopy grids for improved cellular cryo-electron tomography throughput

Published online by Cambridge University Press:  30 July 2021

Leeya Engel ,
Claudia Vasquez ,
Elizabeth Montabana ,
Belle Sow ,
Marcin Walkiewicz ,
William Weis  and
Alexander Dunn
Leeya Engel
Affiliation:
Stanford University, United States
Claudia Vasquez
Affiliation:
Stanford University, United States
Elizabeth Montabana
Affiliation:
Stanford University, United States
Belle Sow
Affiliation:
Stanford University, United States
Marcin Walkiewicz
Affiliation:
Stanford University, United States
William Weis
Affiliation:
Stanford University School of Medicine, United States
Alexander Dunn
Affiliation:
Stanford University, United States

Abstract

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Type
Cryo-electron Tomography: Present Capabilities and Future Potential
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 2570 - 2573
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Engel, Leeya, Gaietta, Guido, Dow, Liam P, Swift, Mark F, Pardon, Gaspard, Volkmann, Niels, Weis, William I, Hanein, Dorit, and Pruitt, Beth L. Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies. Journal of Micromechanics and Microengineering, 29(11):115018, sep 2019. doi:10.1088/1361- 6439/ab419a.CrossRefGoogle ScholarPubMed
Toro-Nahuelpan, Mauricio, Zagoriy, Ievgeniia, Senger, Fabrice, Blanchoin, Laurent, Théry, Manuel, and Mahamid, Julia.Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies. Nature Methods, 17(1):50–54, 2020.CrossRefGoogle ScholarPubMed