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Mapping the Subcellular Distribution of Calcium in Depolarized Neurons by Electron Energy Loss Spectrum Imaging

Published online by Cambridge University Press:  02 July 2020

S.B. Andrews ,
J. Hongpaisan ,
N.B. Pivovarova ,
D.D. Friel  and
R.D. Leapman
S.B. Andrews
Affiliation:
laboratory of Neurobiology, NINDS, and, Div. Bioengineering & Physical Science, ORS, NIH, Bethesda, MD20892
J. Hongpaisan
Affiliation:
laboratory of Neurobiology, NINDS, and, Div. Bioengineering & Physical Science, ORS, NIH, Bethesda, MD20892
N.B. Pivovarova
Affiliation:
laboratory of Neurobiology, NINDS, and, Div. Bioengineering & Physical Science, ORS, NIH, Bethesda, MD20892
D.D. Friel
Affiliation:
Dept. Neuroscience, Case Western Reserve, Cleveland, OH44106.
R.D. Leapman
Affiliation:
Div. Bioengineering & Physical Science, ORS, NIH, Bethesda, MD20892
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Extract

In the context of biological specimens, it is in principle desirable to quantitatively map, rather than just point analyze, the distribution of physiologically important elements, and to do so at subcellular resolution. Presently, this can be accomplished by electron energy loss spectrum-imaging (EELSI) in both the scanning transmission electron microscope (STEM) and the energy-filtering transmission electron microscope (EFTEM). Until recently, this approach has been of limited value for mapping the particularly important element Ca, mainly because intracellular total Ca concentrations are normally quite low (<5 mmol/kg dry weight) and because the background in the vicinity of the Ca L23 edge is complex and requires precise background modeling to extract the very weak Ca signals. As a result, the Ca signal is usually not high enough to reach detection threshold during a practical EELSI acquisition time.

Type
Electron Energy-Loss Spectroscopy (EELS) and Imaging
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 162 - 163
Copyright
Copyright © Microscopy Society of America

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