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LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs

Published online by Cambridge University Press:  05 September 2022

Aydın Alan ,
Emel Alan Open the ORCID record for Emel Alan [Opens in a new window] ,
Korhan Arslan Open the ORCID record for Korhan Arslan [Opens in a new window] ,
Fadime Daldaban ,
Esma Gamze Aksel ,
Mehmet Ulaş Çınar  and
Bilal Akyüz
Aydın Alan
Affiliation:
Department of Anatomy, Faculty of Veterinary Medicine, Erciyes University, 38030 Kayseri, Turkey
Emel Alan
Affiliation:
Department of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, 38030 Kayseri, Turkey
Korhan Arslan*
Affiliation:
Department of Genetics, Faculty of Veterinary Medicine, University of Erciyes, 38030 Kayseri, Turkey
Fadime Daldaban
Affiliation:
Department of Genetics, Faculty of Veterinary Medicine, University of Erciyes, 38030 Kayseri, Turkey
Esma Gamze Aksel
Affiliation:
Department of Genetics, Faculty of Veterinary Medicine, University of Erciyes, 38030 Kayseri, Turkey
Mehmet Ulaş Çınar
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Erciyes, 38030 Kayseri, Turkey Department of Veterinary Microbiology & Pathology, Washington State University, Pullman, WA 99164, USA
Bilal Akyüz
Affiliation:
Department of Genetics, Faculty of Veterinary Medicine, University of Erciyes, 38030 Kayseri, Turkey
*
*Corresponding author: Korhan Arslan, E-mail: [email protected]
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Abstract

Toll-like receptor (TLR)-mediated inflammatory processes play a critical role in the innate immune response during the initial interaction between the infecting microorganism and immune cells. This study aimed to investigate the possible microanatomical and histological differences in mandibular and bronchial lymph nodes in Akkaraman and Romanov lambs induced by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and study the gene, protein, and immunoexpression levels of TLR4, myeloid differentiation factor 88 (MyD88), and tumor necrosis factor-α (TNF-α) that are involved in the immune system. Microanatomical examinations demonstrated more intense lymphocyte infiltration in the bronchial lymph nodes of Akkaraman lambs in the LPS and LTA groups compared to Romanov lambs. TLR4, MyD88, and TNF-α immunoreactivities were more intense in the experimental groups of both breeds. Expression levels of MyD88 and TNF-α genes in the bronchial lymph node of Akkaraman lambs were found to increase statistically significantly in the LTA group. TLR4 gene expression level in the mandibular lymph node was found to be statistically significantly higher in the LTA + LPS group. In conclusion, dynamic changes in the immune cell populations involved in response to antigens such as LTA and LPS in the lymph nodes of both breeds can be associated with the difference in the expression level of the TLR4/MyD88/TNF-α genes.

Keywords

gene expressionimmunohistochemistrylamblymph nodescanning electron microscopy (SEM)
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 28 , Issue 6 , December 2022 , pp. 2078 - 2092
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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