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Localization of Z-RNA in Normal Lens Epithelium: Middle Fibers

Published online by Cambridge University Press:  02 July 2020

C.E. Gagna ,
H.R. Kuo ,
R. Schulz ,
R. Cordova ,
G. Crippen  and
W.C. Lambert
C.E. Gagna
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA Department of Life Sciences, NYIT, Old Westury, NY11508USA department of Biology, FDU, Teaneck, NJ07666USA
H.R. Kuo
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA
R. Schulz
Affiliation:
department of Biology, FDU, Teaneck, NJ07666USA
R. Cordova
Affiliation:
Department of Life Sciences, NYIT, Old Westury, NY11508USA
G. Crippen
Affiliation:
Department of Life Sciences, NYIT, Old Westury, NY11508USA
W.C. Lambert
Affiliation:
Department of Pathology, UMDNJ-Medical School, Newark, NJ07103USA
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Extract

The goal of this project was to analyze the cellular localization of Z-RNA, within middle fibers (MF) of the adult dog ocular lens (1.5 yr) (Fig. 1), using anti-Z-RNA IgG polyclonal antibodies. B-DNA can adopt the left-handed Z-DNA conformation in vitro (1). Right-handed A-RNA can be transformed into left-handed Z-RNA (2). Z-RNA has been studied in cultured cells (3). Evidence supports the presence of Z-DNA in vivo (1). Removal of DNA binding proteins by fixatives can initiate supercoiling which stabilizes Z-DNA sequences (1).

Anti-Z-RNA polyclonal antibody probes were developed in rabbits immunized with multiple injections of Z-RNA: Br-poly[ribosomal(G-C)]. Regarding immunohistochemistry, lens tissues were fixed in Carnoy's, embedded in paraffin and sectioned (2.5 μm) (Fig. 2). Computerized image analysis was performed using a Leitz DM-RB microscope and Leica Quantiment 500 + image analyzer.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 900 - 901
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Sinden, R., DNA Structure & Function, NY, Academic Press (1994) 179CrossRefGoogle Scholar
2.Hall, K. et al., Nature 311 (1984) 584CrossRefGoogle Scholar
3.Zarling, D.A. et al., J. Mol. Biol. 211 (1990) 147CrossRefGoogle Scholar
4.Harris, J.R., Electron Microscopy in Biology, Oxford, IRL Press (1991) 59Google Scholar
5.Gagna, C.E. et al., J. Histochem. Cytochem. 45 (1997) 1511CrossRefGoogle Scholar
6.Gagna, C.E. et al., Scanning. 20 (1998) 255Google Scholar