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Laser Capture Microdissection, Amplified Antisense Rna, and Highdensity Microarrays: The New Frontier for Gene Expression Profiling From Limited Amounts of Tissue

Published online by Cambridge University Press:  02 July 2020

Roger D. Madison, Ph.D.
Roger D. Madison, Ph.D.*
Affiliation:
Division of Neurosurgery, Duke University Medical Center, and Veterans Affairs Medical Center, Durham, NC27710
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Extract

Because the genes in all somatic cells of an individual are identical, it is the differential expression of the proteins encoded by particular genes that determines a cell's phenotype. There is now increasing interest in relating the simultaneous expression patterns of multiple proteins to complex biological processes. The goal is to develop ‘expression profiles’ of the proteins that are active within a cell or tissue during a particular condition. The ultimate control of functional protein expression is quite complex and may involve transcriptional, combinatorial, and postranslational mechanisms, as well as differences in protein degradation rates that vary under different conditions. Although mRNA levels do not always predict functional protein levels, they are a useful first step in determining the expression profile.

The evolution of three key technologies offers the promise of obtaining quantitative analysis of gene expression from restricted amounts of nervous system tissue. Laser capture microdissection can accomplish harvesting of identified cell populations from histologically processed tissue.

Type
Recent Advances in Light Microscopy
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 840 - 841
Copyright
Copyright © Microscopy Society of America

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References

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13. This work supported by NTH (NS22404-13), and the Veterans Affairs Administration. RDM is a Career Research Scientist within the VA Medical Research Service.Google Scholar