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Is the Binding Pattern of Zinc(II) Equal in Different Bryophyte Species?

Part of: Micrographia Collection

Published online by Cambridge University Press:  27 February 2018

Marko S. Sabovljević ,
Marieluise Weidinger ,
Aneta D. Sabovljević ,
Wolfram Adlassnig  and
Ingeborg Lang
Marko S. Sabovljević*
Affiliation:
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Marieluise Weidinger
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
Aneta D. Sabovljević
Affiliation:
Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Wolfram Adlassnig
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
Ingeborg Lang
Affiliation:
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
*
Author for correspondence: Marko S. Sabovljević, E-mail: [email protected]
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Abstract

Bryophytes are usually taken as good bioindicators. However, they represent a large group of terrestrial plants and they express an enormous range of peculiarities within the plant kingdom. With the aim to search for a common pattern of zinc binding, we established axenical in vitro cultures of a dozen bryophyte species that include hornworts, thallose, and leafy liverworts, as well as acrocarp and pleurocarp mosses. The species were grown free of contaminants for many years prior to the application of different treatments, i.e. offering Zn(II) from solid and liquid media and in combination with different anions. The localization and binding of zinc was detected by confocal microscopy using the zinc-specific dye FluoZin™-3. In one of the species, Hypnum cupressiforme (which is widely used for atmospheric heavy metal deposition studies in biomonitoring), semi-quantitative analyses of zinc were performed by energy dispersive X-ray microspectrometry (EDX) in a scanning electron microscope. The results suggest no common pattern of Zn(II) binding in different bryophyte species. Instead, the binding pattern seems to be species specific. Zinc is located in certain areas or cellular compartments, as clearly shown by the EDX measurements in H. cupressiforme.

Keywords

bryophyteszincuptakeconfocal microscopyX-ray microanalysis
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 24 , Issue 1 , February 2018 , pp. 69 - 74
Copyright
© Microscopy Society of America 2018 

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References

Baker, AJM (1981) Accumulators and excluders – Strategies in the response of plants to heavy-metals. J Plant Nutr 3, 643–654.Google Scholar
Boquete, MT, Fernandez, JA, Aboal, JR and Carballeira, A (2011) Are terrestrial mosses good biomonitors of atmospheric deposition of Mn? Atmos Environ 45, 2704–2710.CrossRefGoogle Scholar
Broadley, MR, White, PJ, Hammond, JP, Zelko, I and Lux, A (2007) Zinc in plants. New Phytologist 173, 677–702.CrossRefGoogle ScholarPubMed
Burton, MAS and Peterson, PJ (1979) Studies on zinc localization in aquatic bryophytes. Bryologist 82, 594–598.CrossRefGoogle Scholar
Czarnowska, K and Rejment-Grochowska, I (1974) Concentration of heavy metals – Iron, manganase, zinc and copper in mosses. Acta Soc Bot Pol 43, 39–44.Google Scholar
Mousavi, SR, Galavi, M and Rezaei, M (2012) The interaction of zinc with other elements in plants: A review. Int J Agric Crop Sci 4, 1881–1884.Google Scholar
Sabovljevic, M, Bijelovic, A and Dragicevic, I (2003) In vitro culture of mosses: Aloina aloides (K.F. Schultz) Kindb., Brachythecium velutinum (Hedw.) B.S.&G., Ceratodon purpureus (Hedw.) Brid., Eurhynchium praelongum (Hedw.) B.S.&G. and Grimmia pulvinata (Hedw.) Sm. Turk J Bot 27, 441–446.Google Scholar
Sabovljevic, A, Cvetic, T and Sabovljevic, M (2006) The establishment and development of the Catherine’s moss Atrichum undulatum (Hedw.) P.Beauv. (Polytrichaceae) in in vitro conditions. Arch Biol Sci 58, 87–93.Google Scholar
Sabovljevic, M, Papp, B, Sabovljevic, A, Vujicic, M, Szurdoki, E and Segarra-Moragues, JG (2012) In vitro micropropagation of rare and endangered moss Enthostodon hungaricus (Funariaceae). Biosci J 28, 632–640.Google Scholar
Sabovljevic, MS, Sabovljevic, AD, Ikram, NKK, Peramuna, A, Bae, H and Simonsen, HT (2016) Bryophytes – An emerging source for herbal remedies and chemical production. Plant Gen Res 14, 314–327.Google Scholar
Sabovljevic, A, Sabovljevic, M and Jockovic, N (2009) In vitro cultureand secondary metaboliteisolationin bryophytes. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Mohan JS and Saxena PK (Eds.), Methods in Molecular Biology, pp. 117–128. New York: Humana Press.Google Scholar
Sabovljevic, M, Vujicic, M, Pantovic, J and Sabovljevic, A (2014) Bryophyte conservation biology: In vitro approach to the ex situ conservation of bryophytes from Europe. Plant Biosys 148, 857–868.Google Scholar
Sassmann, S, Adlassnig, W, Puschenreiter, M, Palomino Cadenas, EJ, Leyvas, M, Lichtscheidl, IK and Lang, I (2015a) Free metal ion availability is a major factor for tolerance and growth in Physcomitrella patens . Environ Exp Bot 110, 1–10.Google Scholar
Sassmann, S, Weidinger, M, Adlassnig, W, Hofhansl, F, Bock, B and Lang, I (2015b) Zinc and copper uptake in Physcomitrella patens: Limitations and effects on growth and morphology. Environ Exp Bot 118, 12–20.Google Scholar
Shaw, J, Antonovics, J and Anderson, LE (1987) Inter- and intraspecific variation of mosses in tolerance to copper and zinc. Evolution 41, 1312–1325.Google Scholar
Tremper, AH, Burton, MAS and Higgs, DEB (2004) Field and laboratory exposures of two moss species to low level metal pollution. J Atmos Chem 49, 111–120.Google Scholar
Tyler, G (1990) Bryophytes and heavy metals: A literature review. Bot J Linn Soc 104, 231–253.Google Scholar
Vujicic, M, Sabovljevic, A and Sabovljevic, M (2011) Axenically culturing the bryophytes: Establishment and propagation of the moss Hypnum cupressiforme Hedw. (Bryophyta, Hypnaceae) in in vitro conditions. Botanica Serbica 35, 71–77.Google Scholar