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The Intriguing Ultrastructure of Lipid Body Organelles Within Activated Macrophages

Published online by Cambridge University Press:  01 May 2014

Felipe F. Dias ,
Victor C. Zarantonello ,
Gleydes G. Parreira ,
Hélio Chiarini-Garcia  and
Rossana C. N. Melo
Felipe F. Dias
Affiliation:
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora (UFJF), Juiz de Fora, MG 36036-900, Brazil
Victor C. Zarantonello
Affiliation:
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora (UFJF), Juiz de Fora, MG 36036-900, Brazil
Gleydes G. Parreira
Affiliation:
Laboratory of Structural Biology and Reproduction, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG 31270-901, Brazil
Hélio Chiarini-Garcia
Affiliation:
Laboratory of Structural Biology and Reproduction, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG 31270-901, Brazil
Rossana C. N. Melo*
Affiliation:
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora (UFJF), Juiz de Fora, MG 36036-900, Brazil
*
*Corresponding author. [email protected]
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Abstract

Macrophages are widely distributed immune system cells with essential functions in tissue homeostasis, apoptotic cell clearance, and first defense in infections. A distinguishing feature of activated macrophages participating in different situations such as inflammatory and metabolic diseases is the presence of increased numbers of lipid-rich organelles, termed lipid bodies (LBs) or lipid droplets, in their cytoplasm. LBs are considered structural markers of activated macrophages and are involved in different functions such as lipid metabolism, intracellular trafficking, and synthesis of inflammatory mediators. In this review, we revisit the distinct morphology of LB organelles actively formed within macrophages in response to infections and cell clearance, taking into account new insights provided by ultrastructural studies. We also discuss the LB interactions within macrophages, revealed by transmission electron microscopy, with a focus on the remarkable LB–phagosome association and discuss potential links between structural aspects and function.

Keywords

lipid dropletscell activationhost-pathogen interactionsinflammationlipid mediators
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 3 , June 2014 , pp. 869 - 878
Copyright
© Microscopy Society of America 2014 

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